Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

被引：25

作者：

Bojmar, Linda ^{[1
,2
,3
]}

Kim, Han Sang ^{[1
,2
,3
,4
]}

Tobias, Gabriel C. ^{[1
,2
,5
]}

Vatter, Fanny A. Pelissier ^{[1
,2
]}

Lucotti, Serena ^{[1
,2
]}

Gyan, Kofi Ennu ^{[1
,2
,6
]}

Kenific, Candia M. ^{[1
,2
]}

Wan, Zurong ^{[1
,2
,7
]}

Kim, Kyung-A ^{[4
]}

Kim, DooA ^{[4
]}

Hernandez, Jonathan ^{[8
,9
]}

Pascual, Virginia ^{[7
,10
]}

Heaton, Todd E. ^{[11
,12
]}

La Quaglia, Michael P. ^{[11
,12
]}

Kelsen, David ^{[13
]}

Trippett, Tanya M. ^{[11
]}

Jones, David R. ^{[14
]}

Jarnagin, William R. ^{[8
]}

Matei, Irina R. ^{[1
,2
]}

Zhang, Haiying ^{[1
,2
]}

Hoshino, Ayuko ^{[1
,2
,15
]}

Lyden, David ^{[1
,2
]}

机构：

[1] Weill Cornell Med, Childrens Canc & Blood Fdn Labs, Drukier Inst Childrens Hlth, Dept Pediat,Meyer Canc Ctr, New York, NY 10021 USA

[2] Weill Cornell Med, Drukier Inst Childrens Hlth, Dept Cell & Dev Biol, Meyer Canc Ctr, New York, NY 10021 USA

[3] Linkoping Univ, Dept Biomed & Clin Sci, S-58183 Linkoping, Sweden

[4] Yonsei Univ, Severance Biomed Sci Inst, Brain Korea Project 21,Coll Med, Yonsei Canc Ctr,Div Med Oncol,Dept Internal Med,G, Seoul, South Korea

[5] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo, Brazil

[6] Triinst PhD Program Computat Biol & Med, New York, NY USA

[7] Weill Cornell Med, Dept Pediat, New York, NY USA

[8] Mem Sloan Kettering Canc Ctr, Dept Surg, Hepatopancreatobiliary Serv, New York, NY USA

[9] NCI, Surg Oncol Program, NIH, Bethesda, MD USA

[10] Weill Cornell Med, Drukier Inst Childrens Hlth, New York, NY USA

[11] Mem Sloan Kettering Canc Ctr, Dept Pediat, New York, NY USA

[12] Mem Sloan Kettering Canc Ctr, Dept Surg, Pediat Surg Serv, New York, NY USA

[13] Mem Sloan Kettering Canc Ctr, Dept Med, New York, NY USA

[14] Mem Sloan Kettering Canc Ctr, Dept Surg, Thorac Surg Serv, New York, NY USA

[15] Tokyo Inst Technol, Sch Life Sci & Technol, Yokohama, Japan

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 01期

基金：

新加坡国家研究基金会; 瑞士国家科学基金会; 美国国家卫生研究院; 巴西圣保罗研究基金会;

关键词：

EXOSOMES;

D O I：

10.1016/j.xpro.2020.100225

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling.For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).

引用

页数：24

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