Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

被引：47

作者：

Krcmova, Lenka ^{[1
,2
]}

Solichova, Dagmar ^{[1
]}

Melichar, Bohuslav ^{[3
]}

Kasparova, Marketa ^{[1
,2
]}

Plisek, Jiri ^{[1
,2
]}

Sobotka, Lubos ^{[1
]}

Solich, Petr ^{[2
]}

机构：

[1] Univ Hosp, Dept Metab Care & Gerontol, Hradec Kralove 50005, Czech Republic

[2] Charles Univ Prague, Fac Pharm, Dept Analyt Chem, CS-50165 Hradec Kralove, Czech Republic

[3] Palacky Univ, Sch Med, Dept Oncol, CR-77147 Olomouc, Czech Republic

来源：

TALANTA | 2011年 / 85卷 / 03期

关键词：

Neopterin; Kynurenine; Tryptophan; HPLC; Monolithic column; Clinical research; PERFORMANCE LIQUID-CHROMATOGRAPHY; SYSTEMIC IMMUNE ACTIVATION; SOLID-PHASE EXTRACTION; URINARY NEOPTERIN; INDOLEAMINE 2,3-DIOXYGENASE; VITAMIN-A; OVARIAN; PROLIFERATION; LYMPHOCYTES; TOCOPHEROL;

D O I：

10.1016/j.talanta.2011.06.027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1466 / 1471

页数：6

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