Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

被引:11
作者
Vokurková, M
Nováková, O
Dobesová, Z
Kunes, J
Zicha, J
机构
[1] Acad Sci Czech Republ, Inst Physiol, CR-14220 Prague, Czech Republic
[2] Cardiovasc Res Ctr, Prague, Czech Republic
[3] Charles Univ, Fac Sci, Dept Anim Physiol & Dev Biol, Prague, Czech Republic
关键词
ion transport; membrane lipids; red blood cells; salt hypertension;
D O I
10.1016/j.lfs.2005.03.014
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Distinct changes of membrane lipid content could contribute to the abnormalities of ion transport that take part in the development of salt hypertension in Dahl rats. The relationships between lipid content and particular ion transport systems were studied in red blood cells (RBC) of Dahl rats kept on low- and high-salt diets for 5 weeks since weaning. Dahl salt-sensitive (SS/Jr) rats on high-salt diet had increased blood pressure, levels of plasma triacylglycerols and total plasma cholesterol compared to salt-resistant (SR/Jr) rats. Furthermore, RBC of SS/Jr rats differed from SR/Jr ones by increased content of total membrane phospholipids, but membrane cholesterol was not changed significantly. SS/Jr rats had higher RBC intracellular Na+ (Na-i(+)) content and enhanced bumetanide-sensitive Rb+ uptake. RBC membrane content of cholesterol and phospholipids correlated positively with RBC Na-i(+) content, with the activity of Na+-K+ pump and Na+-K+-2Cl(-) cotransport and also with Rb+ leak. The content of phosphatidylserines plus phosphatidylinositols was positively associated with RBC Na-i(+) content, with the activity of Na+-K+ pump and Na+-K+-2Cl(-) cotransport and with Rb+ leak. The content of sphingomyelins was positively related to Na+-K+-2Cl(-) cotransport activity and negatively to ouabain-sensitive Rb+-K+ exchange. We can conclude that observed relationships between ion transport and the membrane content of cholesterol and/or sphingomyelins, which are known to regulate membrane fluidity, might participate in the pathogenesis of salt hypertension in Dahl rats. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:1452 / 1464
页数:13
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