Dual-vectors of anti-cancer drugs and genes based on pH-sensitive micelles self-assembled from hybrid polypeptide copolymers

被引：40

作者：

Li, Yong-Yong ^{[1
,2
,3
]}

Hua, Shou-Hu ^{[1
,2
]}

Xiao, Wang ^{[1
,2
]}

Wang, Hui-Yuan ^{[1
,2
]}

Luo, Xiao-Hua ^{[1
,2
]}

Li, Cao ^{[1
,2
]}

Cheng, Si-Xue ^{[1
,2
]}

Zhang, Xian-Zheng ^{[1
,2
]}

Zhuo, Ren-Xi ^{[1
,2
]}

机构：

[1] Wuhan Univ, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[3] Tongji Univ, Inst Adv Mat & Nano Biomed iNANO, Shanghai 200092, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY | 2011年 / 21卷 / 09期

关键词：

POLY-L-LYSINE; BLOCK-COPOLYMER; TRANSFECTION EFFICIENCY; BIOLOGICAL SIGNIFICANCE; MULTIBLOCK COPOLYMERS; POLY(ETHYLENE OXIDE); AQUEOUS-SOLUTION; DELIVERY; DNA; ACID);

D O I：

10.1039/c0jm03385e

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A series of amphiphilic pH-sensitive hybrid polypeptide copolymers, poly(ethylene glycol)-b-poly-(L-lysine)-b-poly(L-phenylalanine) (PEG-PLL-PLP) were synthesized. The copolymers could self-assemble into micelles with PLP as the hydrophobic core and PEG-PLL as the hydrophilic shell, as evidenced by (HNMR)-H-1 and TEM. These micelles exhibited obvious pH response in hydrodynamic diameter and pH-dependent drug release behavior, attributed to the protonation/deprotonation of amino groups in PLL segments. The copolymers could further condense plasmid DNA efficiently. Importantly, the polymer/DNA complexes showed high transfection efficiency in 293T cells under optimized conditions. This study suggested the copolymers may have great potential in both drug and gene delivery.

引用

页码：3100 / 3106

页数：7

共 30 条

[1] Synthesis of biodegradable multi-block copolymers of poly(L-lysine) and poly(ethylene glycol) as a non-viral gene carrier [J].