Protein adsorption behaviors of carboxymethylated bacterial cellulose membranes

被引：43

作者：

Lin, Qinghua ^{[1
]}

Zheng, Yudong ^{[1
]}

Wang, Guojie ^{[1
]}

Shi, Xiangning ^{[1
]}

Zhang, Tao ^{[1
]}

Yu, Jie ^{[1
]}

Sun, Jian ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 73卷

基金：

中国国家自然科学基金;

关键词：

Carboxymethylated; Bacterial cellulose; Surface; Protein adsorption; MICROBIAL CELLULOSE; MECHANICAL-PROPERTIES; NANOCOMPOSITES;

D O I：

10.1016/j.ijbiomac.2014.11.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carboxymethylated bacterial cellulose (CBC) membranes with different degrees of substitution (DS) were prepared by surface carboxymethylation of bacterial cellulose, which were characterized by FTIR, contact angles and zeta potential. Both the contact angels and zeta potentials increased with the increase of degrees of carboxymethylation. Protein adsorption behaviors of CBC membranes were investigated with bovine serum albumin (BSA) as model protein and the adsorption quantity increased gradually with the increase of the DS. The protein adsorption was not only governed by the chemical structure of CBC, but also by the electrostatic interaction between CBC and BSA. The protein was found to be adsorbed on CBC membrane at pH lower its isoelectric point (pI) while no protein was adsorbed at pH above its pI. The protein adsorption on the CBC membranes was also characterized by scanning electron microscopy (SEM). (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：264 / 269

页数：6

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