3D Imaging of Nanoparticle Distribution in Biological Tissue by Laser-Induced Breakdown Spectroscopy

被引：97

作者：

Gimenez, Y. ^{[1
]}

Busser, B. ^{[1
]}

Trichard, F. ^{[1
]}

Kulesza, A. ^{[1
]}

Laurent, J. M. ^{[2
]}

Zaun, V. ^{[3
]}

Lux, F. ^{[1
]}

Benoit, J. M. ^{[1
]}

Panczer, G. ^{[1
]}

Dugourd, P. ^{[1
]}

Tillement, O. ^{[1
]}

Pelascini, F. ^{[3
]}

Sancey, L. ^{[1
]}

Motto-Ros, V. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, UMR5306, Inst Lumiere Mat, F-69622 Villeurbanne, France

[2] Andor Technol, Springvale Business Pk, Belfast BT12 7AL, Antrim, North Ireland

[3] CRITT Mat Alsace, F-67305 Schiltigheim, France

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

GADOLINIUM-BASED NANOPARTICLES; MASS-SPECTROMETRY; TUMOR; DELIVERY; RESOLUTION; METALS; CELLS;

D O I：

10.1038/srep29936

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nanomaterials represent a rapidly expanding area of research with huge potential for future medical applications. Nanotechnology indeed promises to revolutionize diagnostics, drug delivery, gene therapy, and many other areas of research. For any biological investigation involving nanomaterials, it is crucial to study the behavior of such nano-objects within tissues to evaluate both their efficacy and their toxicity. Here, we provide the first account of 3D label-free nanoparticle imaging at the entire-organ scale. The technology used is known as laser-induced breakdown spectroscopy (LIBS) and possesses several advantages such as speed of operation, ease of use and full compatibility with optical microscopy. We then used two different but complementary approaches to achieve 3D elemental imaging with LIBS: a volume reconstruction of a sliced organ and in-depth analysis. This proof-of-concept study demonstrates the quantitative imaging of both endogenous and exogenous elements within entire organs and paves the way for innumerable applications.

引用

页数：9

共 45 条

[41] Label-Free Au Cluster Used for in Vivo 2D and 3D Computed Tomography of Murine Kidneys [J].

Wang, Yaling ;

Xu, Chao ;

Zhai, Jiao ;

Gao, Fuping ;

Liu, Ru ;

Gao, Liang ;

Zhao, Yiliang ;

Chai, Zhifang ;

Gao, Xueyun .

ANALYTICAL CHEMISTRY, 2015, 87 (01) :343-345

[42] Imaging mass spectrometry in microbiology [J].

Watrous, Jeramie D. ;

Dorrestein, Pieter C. .

NATURE REVIEWS MICROBIOLOGY, 2011, 9 (09) :683-694

[43] Martian Fluvial Conglomerates at Gale Crater [J].

Williams, R. M. E. ;

Grotzinger, J. P. ;

Dietrich, W. E. ;

Gupta, S. ;

Sumner, D. Y. ;

Wiens, R. C. ;

Mangold, N. ;

Malin, M. C. ;

Edgett, K. S. ;

Maurice, S. ;

Forni, O. ;

Gasnault, O. ;

Ollila, A. ;

Newsom, H. E. ;

Dromart, G. ;

Palucis, M. C. ;

Yingst, R. A. ;

Anderson, R. B. ;

Herkenhoff, K. E. ;

Le Mouelic, S. ;

Goetz, W. ;

Madsen, M. B. ;

Koefoed, A. ;

Jensen, J. K. ;

Bridges, J. C. ;

Schwenzer, S. P. ;

Lewis, K. W. ;

Stack, K. M. ;

Rubin, D. ;

Kah, L. C. ;

Bell, J. F., III ;

Farmer, J. D. ;

Sullivan, R. ;

Van Beek, T. ;

Blaney, D. L. ;

Pariser, O. ;

Deen, R. G. .

SCIENCE, 2013, 340 (6136) :1068-1072

[44] Trace Metals as Biomarkers for Eumelanin Pigment in the Fossil Record [J].

Wogelius, R. A. ;

Manning, P. L. ;

Barden, H. E. ;

Edwards, N. P. ;

Webb, S. M. ;

Sellers, W. I. ;

Taylor, K. G. ;

Larson, P. L. ;

Dodson, P. ;

You, H. ;

Da-Qing, L. ;

Bergmann, U. .

SCIENCE, 2011, 333 (6049) :1622-1626

[45] Comparative imaging of P, S, Fe, Cu, Zn and C in thin sections of rat brain tumor as well as control tissues by laser ablation inductively coupled plasma mass spectrometry [J].

Zoriy, Myroslav V. ;

Dehnhardt, Markus ;

Matusch, Andreas ;

Becker, J. Sabine .

SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2008, 63 (03) :375-382

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