Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype

被引：21
|
作者：
Zhou, Xiaopeng [1 ]
Tao, Yiqing [1 ]
Wang, Jin [1 ]
Liang, Chengzhen [1 ]
Wang, Jun [1 ]
Li, Hao [1 ]
Chen, Qixin [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou 310009, Zhejiang, Peoples R China
来源：
GROWTH FACTORS | 2015年 / 33卷 / 01期
关键词：
Differentiation; fibroblast growth factor-2; mesenchymal stem cells; nucleus pulposus-like phenotype; transforming growth factor-beta; FIBROBLAST-GROWTH-FACTOR; INTERVERTEBRAL DISC REGENERATION; IN-VITRO; SIGNALING PATHWAYS; TRANSPLANTATION THERAPY; ARTICULAR-CARTILAGE; EXPRESSION; PROLIFERATION; ACTIVATION; ERK;
D O I：
10.3109/08977194.2014.969420
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Human mesenchymal stem cells (MSCs) are reported to have the capability of differentiating towards nucleus pulposus (NP)-like phenotype under specific culture conditions. So far, the effects of fibroblast growth factor (FGF)-2 and the cocktail effects of transforming growth factor (TGF)-beta and FGF-2 on MSCs remain unclear. Therefore, we designed this study to clarify these effects. MSCs were cultured in conditioned medium containing FGF-2 or TGF-beta/FGF-2, and compared with basal or TGF-beta medium. The groups with FGF-2 showed the increase of cell proliferation. Functional gene markers and novel NP markers decreased in FGF-2 group, together with functional protein expression. Pho-ERK1/2 and pho-Smad3 differed significantly in the two conditioned groups. All these results suggest FGF-2 promotes MSCs' proliferation, synergistically with TGF-beta. However, FGF-2 plays a negative role in cartilage homeostasis. We also demonstrate that FGF-2 has no positive effect in differentiating MSCs into NP-like cells, but hinders the acceleration effect of TGF-beta.
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页码：23 / 30
页数：8
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