High-performance thin-layer chromatographic analysis of three components in Arnebia guttata Bunge

被引:0
作者
Yan, Xiaocai [1 ]
Ding, Wenhuan [2 ]
Xu, Haiyan [1 ]
机构
[1] Xinjiang Med Univ, Coll TCM, Urumqi 830011, Xinjiang, Peoples R China
[2] Xinjiang Med Univ, Urumqi 830011, Xinjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Arnebia guttata Bunge; beta-Acetoxyisovalerylalkannin; Acetylshikonin; beta; beta'-Dimethylacrylalkannin; High-performance thin-layer chromatography; Qualitative and quantitative analysis;
D O I
10.1007/s00764-021-00143-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High-performance thin-layer chromatography (HPTLC) was used for the qualitative and quantitative analysis of beta-acetoxyisovalerylalkannin, acetylshikonin and beta,beta'-dimethylacrylalkannin in Arnebia guttata Bunge. The mobile phase used was petroleum ether (60-90 degrees C): xylene-ethyl acetate-formic acid (8:3:0.5:1, V/V). TLC plate was dried at 105 degrees C for 30 min and applied on plate. The data used were obtained by measuring at 522 nm. Results showed that beta-acetoxyisovalerylalkannin, acetylshikonin and beta,beta'-dimethylacrylalkannin had R-F values of 0.31 +/- 0.05, 0.46 +/- 0.05 and 0.64 +/- 0.05, respectively. The linear ranges were 0.2002-1.203, 0.076-0.760 and 0.201-1.206 mu g/band. The limits of detection were 37.5, 50 and 37.5 ng/band. The limits of quantification were 125, 167 and 125 ng/band. The average recoveries were 100.69%, 98.90% and 100.06%. The relative standard deviation was less than 5%. In conclusion, three components of A. guttata Bunge could be isolated and quickly detected by HPTLC which can lay a foundation for identification.
引用
收藏
页码:503 / 512
页数:10
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