Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination

被引：10

作者：

Kenny, Grace ^{[1
,2
]}

Negi, Riya ^{[1
]}

O'Reilly, Sophie ^{[1
]}

Garcia-Leon, Alejandro ^{[1
]}

Alalwan, Dana ^{[1
]}

Gaillard, Colette Marie ^{[1
]}

Saini, Gurvin ^{[1
]}

Inzitari, Rosana ^{[3
]}

Feeney, Eoin R. ^{[1
,2
,7
]}

Yousif, Obada ^{[4
]}

Cotter, Aoife G. ^{[1
,5
]}

de Barra, Eoghan ^{[6
]}

Sadlier, Corinna ^{[8
]}

Crispie, Fiona ^{[9
,10
]}

Doran, Peter ^{[3
]}

Gautier, Virginie ^{[1
]}

Mallon, Patrick W. G. ^{[1
,2
]}

机构：

[1] Univ Coll Dublin, Ctr Expt Pathogen Host Res CEPHR, Dublin 4, Ireland

[2] St Vincents Univ Hosp, Dept Infect Dis, Elm Pk, Dublin 4, Ireland

[3] Univ Coll Dublin, Sch Med, Dublin 4, Ireland

[4] Wexford Gen Hosp, Endocrinol Dept, Wexford, Ireland

[5] Mater Misericordiae Univ Hosp, Dept Infect Dis, Eccles St, Dublin 7, Ireland

[6] Beaumont Hosp, Dept Infect Dis, Dublin 9, Ireland

[7] Royal Coll Surgeons Ireland, Dept Int Hlth & Trop Med, Dublin, Ireland

[8] Cork Univ Hosp, Dept Infect Dis, Cork, Cork, Ireland

[9] Teagasc Food Res Ctr, Moorepk, Ireland

[10] APC Microbiome Ireland, Cork, Ireland

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2022年 / 510卷

基金：

爱尔兰科学基金会;

关键词：

SARS-CoV-2; COVID-19; Serology; ANTIBODIES;

D O I：

10.1016/j.jim.2022.113345

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8-3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen.

引用

页数：8

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