Inhibitory effect of chitinases isolated from Semillon grapes (Vitis vinifera) on growth of grapevine pathogens

被引：14

作者：

Saito, Seiya ^{[1
]}

Odagiri, Masanori ^{[1
]}

Furuya, Seiichi ^{[1
]}

Suzuki, Shunji ^{[1
]}

Takayanagi, Tsutomu ^{[1
]}

机构：

[1] Univ Yamanashi, Inst Enol & Viticulture, Lab Fruit Genet Engn, Yamanashi 4000005, Japan

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 20卷 / 01期

关键词：

Chitinase; Grapevine; Vitis vinifera; Botrytis cinerea; Glomerella cingulata; ANTIFUNGAL ACTIVITY; BOTRYTIS-CINEREA; SALICYLIC-ACID; PROTEINS; FUNGAL; BETA-1,3-GLUCANASE; EXPRESSION; RESISTANCE; LEAVES; INFECTION;

D O I：

10.1007/s13562-010-0025-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Characteristics and antifungal activity of chitinases in Semillon grapes were investigated. Chitinases were isolated from the juice of Semillon grapes by chitin affinity chromatography. Native and SDS-PAGE analyses of the fraction showing chitin affinity (active fraction) demonstrated only the presence of protein bands of chitinases. Three types of class IV chitinases (chi-la, chi-1b and chi-2) were purified from the active fraction. These chitinases actively hydrolyzed chitin under acidic conditions (pH 4.0-4.5). The isoelectric points and the molecular weights of chi-la, chi-1b and chi-2 were 4.73, 4.60, and 7.87, and 32.1 kDa, 31.6 kDa, and 29.0 kDa, respectively. The active fraction was found to inhibit Botrytis cinerea mycelial growth and the inhibitory effect was due to the activity of chitinases. The active fraction inhibited twenty strains of B. cinerea collected from the experimental vineyard. The effect of chitinases was enhanced in media containing more than 20% sugar. When the active fraction was tested on Glomerella cingulata, the growth inhibitory effect observed was markedly less than that seen on B. cinerea.

引用

页码：47 / 54

页数：8

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