Optimization of culture medium and conditions for neo-fructooligosaccharides production by Penicillium citrinum

被引：35

作者：

Lim, JS

Park, MC

Lee, JH

Park, SW

Kim, SW

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] CJ Corp, CJ Foods R&D, Food Ingredient Div, Seoul 152050, South Korea

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2005年 / 221卷 / 05期

基金：

欧洲研究理事会;

关键词：

optimization; response surface methodology; neo-fructooligosaccharide; Penicillium citrinum; statistical analysis;

D O I：

10.1007/s00217-005-0070-6

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Culture medium and conditions were optimized to improve neo-fructooilgosaccharide (neo-FOS) production by Penicillium citrinum using Response Surface Methodology (RSM). The central composite designs using RSM were employed in this investigation. The quadratic models for neo-FOS production were obtained. Among culture media, optimal concentration of sucrose and yeast extract were found to be 12.65% and 2.90%, respectively, and predicted maximum value of neo-FOS production was 39.3 g/l. For culture conditions, optimal points of inoculum age (78.5 h), initial pH (7.34) and inoculum size (11.36% (v/v)) were determined and predicted maximum value of neo-FOS production was 40.6 g/l. By performing the culture under optimal culture media and conditions, actual neo-FOS production increased to 107%. It was also suggested that RSM can be used as a potential tool for optimization of factors in fermentation and food industry.

引用

页码：639 / 644

页数：6

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