Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells

被引：32

作者：

Akutsu, Hidenori ^{[1
]}

Machida, Masakazu ^{[1
]}

Kanzaki, Seiichi ^{[1
]}

Sugawara, Tohru ^{[1
]}

Ohkura, Takashi ^{[1
]}

Nakamura, Naoko ^{[1
]}

Yamazaki-Inoue, Mayu ^{[1
]}

Miura, Takumi ^{[1
]}

Vemuri, Mohan C. ^{[2
]}

Rao, Mahendra S. ^{[3
]}

Miyado, Kenji ^{[1
]}

Umezawa, Akihiro ^{[1
]}

机构：

[1] Natl Res Inst Child Hlth & Dev, Dept Reprod Biol, Ctr Regenerat Med, Setagaya Ku, 2-10-1 Okura, Tokyo 1578535, Japan

[2] Thermo Fisher Sci, Frederick, MD 21702 USA

[3] NIH, Ctr Regenerat Med, Bethesda, MD 20892 USA

来源：

REGENERATIVE THERAPY | 2015年 / 1卷

关键词：

Human embryonic stem cells; Xenogeneic-free medium; Stem cell expansion; Human feeder layer; Mesenchymal stem cells; Gamma irradiation;

D O I：

10.1016/j.reth.2014.12.004

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and -7). Four of them were derived and maintained on irradiated human mesenchymal stem cells (hMSCs) grown in xenogeneic-free defined media and substrate. Xenogeneic-free hMSCs isolated from the subcutaneous tissue of extra fingers from individuals with polydactyly showed appropriate potentials as feeder layers in the pluripotency and growth of hESCs. In this report, we describe a comprehensive characterization of these newly derived SEES cell lines. In addition, we developed a scalable culture system for hESCs having high biological safety by using gamma-irradiated serum replacement and pharmaceutical-grade recombinant basic fibroblast growth factor (bFGF, also known as trafermin). This is first report describing the maintenance of hESC pluripotency using pharmaceutical-grade human recombinant bFGF (trafermin) and gamma-irradiated serum replacement. Our defined medium system provides a path to scalability in Good Manufacturing Practice (GMP) settings for the generation of clinically relevant cell types from pluripotent cells for therapeutic applications. (C) 2015, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. All rights reserved.

引用

页码：18 / 29

页数：12

共 32 条

[1] Human embryonic stem cells [J].

Akutsu, Hidenori ;

Cowan, Chad A. ;

Melton, Douglas .

EMBRYONIC STEM CELLS, 2006, 418 :78-92

[2] Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture [J].

Amit, M ;

Carpenter, MK ;

Inokuma, MS ;

Chiu, CP ;

Harris, CP ;

Waknitz, MA ;

Itskovitz-Eldor, J ;

Thomson, JA .

DEVELOPMENTAL BIOLOGY, 2000, 227 (02) :271-278

[3] Optimal Timing of Inner Cell Mass Isolation Increases the Efficiency of Human Embryonic Stem Cell Derivation and Allows Generation of Sibling Cell Lines [J].

Chen, Alice E. ;

Egli, Dieter ;

Niakan, Kathy ;

Deng, Jie ;

Akutsu, Hidenori ;

Yamaki, Mariko ;

Cowan, Chad ;

Fitz-Gerald, Claire ;

Zhang, Kun ;

Melton, Douglas A. ;

Eggan, Kevin .

CELL STEM CELL, 2009, 4 (02) :103-106

[4]

Cowan CA, 2004, NEW ENGL J MED, V350, P1353, DOI 10.1056/NEJMsr040330

[5] Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy [J].

Cui, Chang-Hao ;

Miyoshi, Shunichiro ;

Tsuji, Hiroko ;

Makino, Hatsune ;

Kanzaki, Seiichi ;

Kami, Daisuke ;

Terai, Masanori ;

Suzuki, Harumi ;

Umezawa, Akihiro .

HUMAN MOLECULAR GENETICS, 2011, 20 (02) :235-244

[6] A Xenogeneic-Free Protocol for Isolation and Expansion of Human Adipose Stem Cells for Clinical Uses [J].

Escobedo-Lucea, Carmen ;

Bellver, Carmen ;

Gandia, Carolina ;

Sanz-Garcia, Andres ;

Esteban, Francisco J. ;

Mirabet, Vicente ;

Forte, Giancarlo ;

Moreno, Isabel ;

Lezameta, Melissa ;

Ayuso-Sacido, Angel ;

Garcia-Verdugo, Jose M. .

PLOS ONE, 2013, 8 (07)

[7] A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells [J].

Fraga, Ana Maria ;

Souza de Araujo, Erica Sara ;

Stabellini, Raquel ;

Vergani, Naja ;

Pereira, Lygia V. .

STEM CELL REVIEWS AND REPORTS, 2011, 7 (04) :775-781

[8] Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues [J].

Hanna, Jacob H. ;

Saha, Krishanu ;

Jaenisch, Rudolf .

CELL, 2010, 143 (04) :508-525

[9] Current Technology for the Derivation of Pluripotent Stem Cell Lines from Human Embryos [J].

Hasegawa, Kouichi ;

Pomeroy, Jordan E. ;

Pera, Martin F. .

CELL STEM CELL, 2010, 6 (06) :521-531

[10] Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression [J].

Hedlund, Maria ;

Padler-Karavani, Vered ;

Varki, Nissi M. ;

Varki, Ajit .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (48) :18936-18941

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