DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

被引：19

作者：

Shah, Pratik ^{[1
]}

Choi, Suk Won ^{[2
]}

Kim, Ho-jin ^{[2
]}

Cho, Seok Keun ^{[1
]}

Thulstrup, Peter Waaben ^{[3
]}

Bjerrum, Morten Jannik ^{[3
]}

Bhang, Yong-Joo ^{[2
]}

Ahn, Jong Cheol ^{[2
]}

Yang, Seong Wook ^{[1
]}

机构：

[1] Univ Copenhagen, UNIK Ctr Synthet Biol, DK-1871 Copenhagen C, Denmark

[2] Seoulin Biosci Co Ltd, Seongnam Si, Gyeonggi Do, South Korea

[3] Univ Copenhagen, Dept Chem, DK-2100 Copenhagen, Denmark

来源：

ANALYST | 2015年 / 140卷 / 10期

关键词：

PANCREATIC-CANCER CELLS; TO-MESENCHYMAL TRANSITION; BREAST-CANCER; RNA/DNA OLIGONUCLEOTIDES; COLORECTAL-CANCER; EXPRESSION; RNA; MIR-200; LET-7A; PROBES;

D O I：

10.1039/c5an00093a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.

引用

页码：3422 / 3430

页数：9

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