DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

被引：19
作者：
Shah, Pratik [1 ]
Choi, Suk Won [2 ]
Kim, Ho-jin [2 ]
Cho, Seok Keun [1 ]
Thulstrup, Peter Waaben [3 ]
Bjerrum, Morten Jannik [3 ]
Bhang, Yong-Joo [2 ]
Ahn, Jong Cheol [2 ]
Yang, Seong Wook [1 ]
机构：
[1] Univ Copenhagen, UNIK Ctr Synthet Biol, DK-1871 Copenhagen C, Denmark
[2] Seoulin Biosci Co Ltd, Seongnam Si, Gyeonggi Do, South Korea
[3] Univ Copenhagen, Dept Chem, DK-2100 Copenhagen, Denmark
来源：
ANALYST | 2015年 / 140卷 / 10期
关键词：
PANCREATIC-CANCER CELLS; TO-MESENCHYMAL TRANSITION; BREAST-CANCER; RNA/DNA OLIGONUCLEOTIDES; COLORECTAL-CANCER; EXPRESSION; RNA; MIR-200; LET-7A; PROBES;
D O I：
10.1039/c5an00093a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.
引用
收藏
页码：3422 / 3430
页数：9
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