Development of a catheter functionalized by a polydopamine peptide coating with antimicrobial and antibiofilm properties

被引:184
作者
Lim, Kaiyang [1 ]
Chua, Ray Rong Yuan [2 ]
Bow, Ho [3 ]
Tambyah, Paul Anantharajah [2 ]
Hadinoto, Kunn [1 ]
Leong, Susanna Su Jan [4 ,5 ,6 ]
机构
[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore
[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore 119228, Singapore
[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 117545, Singapore
[4] Singapore Inst Technol, Singapore 138683, Singapore
[5] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore 117597, Singapore
[6] Natl Univ Singapore, Synthet Biol Res Consortium, Singapore 117456, Singapore
关键词
Antimicrobial peptide; Polydopamine; Urinary catheter; Peptide immobilization; Cytotoxicity; URINARY-TRACT-INFECTIONS; SURFACE MODIFICATION; ANTIBACTERIAL SURFACES; SILVER NANOPARTICLES; CATIONIC PEPTIDES; IMMOBILIZATION; BACTERIA; BIOMATERIALS; ANTIBIOTICS; MECHANISMS;
D O I
10.1016/j.actbio.2014.12.015
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Catheter-associated urinary tract infections (CAUTIs) are the most common hospital-acquired infections worldwide, aggravating the problem of antimicrobial resistance and patient morbidity. There is a need for a potent and robust antimicrobial coating for catheters to prevent these infections. An ideal coating agent should possess high antimicrobial efficacy and be easily and economically conjugated to the catheter surface. In this study, we report a simple yet effective immobilization strategy to tether a potent synthetic antimicrobial peptide, CWR11, onto catheter-relevant surfaces. Polydopamine (PD) was deposited as a thin adherent film onto a polydimethylsiloxane (PDMS) surface to facilitate attachment of CWR11 onto the PD-functionalized polymer. Surface characterization of the CWR11-tethered surfaces confirmed the successful immobilization of peptides onto the PD-coated PDMS. The CWR11-immobilized PDMS slides displayed excellent antimicrobial (significant inhibition of 5 x 10(4) colony-forming units of CAUTI-relevant microbes) and antibiofilm (similar to 92% enhanced antibacterial adherence) properties. To assess its clinical relevance, the PD-based immobilization platform was translated onto commercial silicone-coated Foley catheters. The CWR11-impregnated catheter displayed potent bactericidal properties against both Gram-positive and Gram-negative bacteria, and retained its antimicrobial functionality for at least 21 days, showing negligible cytotoxicity against human erythrocyte and uroepithelial cells. The outcome of this study demonstrates the proof-of-concept potential of a polydopamine-CWR11-fimctionalized catheter to combat CAUTIs. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:127 / 138
页数:12
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