Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity

被引:58
作者
Belen Agueero, Maria
Svetaz, Laura [2 ]
Sanchez, Marianela [3 ]
Luna, Lorena
Lima, Beatriz
Liza Lopez, Maria
Zacchino, Susana [2 ]
Palermo, Jorge [3 ]
Wunderlin, Daniel [4 ]
Egly Feresin, Gabriela
Tapia, Alejandro [1 ]
机构
[1] Univ Nacl San Juan, Inst Biotecnol, Fac Ingn, Inst Ciencias Basicas, RA-5400 San Juan, Argentina
[2] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina
[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, UMYMFOR, RA-1428 Buenos Aires, DF, Argentina
[4] Univ Nacl Cordoba CONICET, Fac Ciencias Quim, Dpto Quim Organ ISIDSA CIBICI Med Allende Esq Hay, RA-5000 Cordoba, Argentina
关键词
Argentinian Andean propolis; Lignans; HPLC-ESI-MS/MS; GC-MS; VOCs; Antifungal activity; CHEMICAL-COMPOSITION; ANTIOXIDANT ACTIVITY; LIGNANS; CONSTITUENTS; MICAFUNGIN; REGIONS; CANDIDA; ORIGIN;
D O I
10.1016/j.fct.2011.05.008
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The chemical profile and botanical origin of Andean Argentinian propolis were studied by HPLC-ESI-MS/MS and GC-MS techniques as well as the antifungal activity according to CLSI protocols. Dermatophytes and yeasts tested were strongly inhibited by propolis extracts (MICs between 31.25 and 125 mu g/mL). The main antifungal compounds were: 3'methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and a NDGA derivative 3, showing strong activity against Trichophyton mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 15.6 and 31.25 mu g/mL). The lignans 1 and 2 showed activities against clinical isolates of Candidas spp., Cryptococcus spp., T. rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.5 mu g/mL). The lignan and volatile organic compounds (VOCs) profiles from propolis matched with those of exudates of Larrea nitida providing strong evidences on its botanical origin. These results support that Argentinian Andean propolis are a valuable natural product with potential to improve human health. Six compounds (1-6) were isolated from propolis for the first time, while compounds 1 and 3-6 were reported for first time as constituents of L nitida Cav. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1970 / 1978
页数:9
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