Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds

被引：1208
作者：
Huangfu, Danwei [1 ]
Maehr, Rene [1 ]
Guo, Wenjun [2 ]
Eijkelenboom, Astrid [1 ,3 ]
Snitow, Melinda [1 ]
Chen, Alice E. [1 ]
Melton, Douglas A. [1 ]
机构：
[1] Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA
[2] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
[3] Univ Utrecht, NL-3508 TC Utrecht, Netherlands
来源：
NATURE BIOTECHNOLOGY | 2008年 / 26卷 / 07期
关键词：
D O I：
10.1038/nbt1418
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase ( HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
引用
收藏
页码：795 / 797
页数：3
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