Deoxynivalenol and nivalenol analysis in soybean and soy flour

A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solid-phase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 mu g/g for DON and 0.2 mu g/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.