A sensitive glucose biosensor based on Ag@C core-shell matrix

被引：37

作者：

Zhou, Xuan ^{[1
]}

Dai, Xingxin ^{[1
]}

Li, Jianguo ^{[1
]}

Long, Yumei ^{[1
,2
]}

Li, Weifeng ^{[1
]}

Tu, Yifeng ^{[1
,2
]}

机构：

[1] Soochow Univ, Chem Engn & Mat Sci, Coll Chem, Suzhou 215123, Jiangsu, Peoples R China

[2] Key Lab Hlth Chem & Mol Diag Suzhou, Suzhou, Peoples R China

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2015年 / 49卷

基金：

中国国家自然科学基金;

关键词：

Ag@C core-shell structure; Glucose oxidase; Biosensor; Electrochemistry; LAYER-BY-LAYER; DIRECT ELECTROCHEMISTRY; SILVER NANOPARTICLES; GOLD NANOPARTICLES; FACILE SYNTHESIS; OXIDASE; CARBON; NANOCOMPOSITE; ELECTRODE; GRAPHENE;

D O I：

10.1016/j.msec.2015.01.063

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Nano-Ag particles were coated with colloidal carbon (Ag@C) to improve its biocompatibility and chemical stability for the preparation of biosensor. The core-shell structure was evidenced by transmission electron microscope (TEM) and the Fourier transfer infrared (FTIR) spectra revealed that the carbon shell is rich of function groups such as OH and COOH. The as-prepared Ag@C core-shell structure can offer favorable microenvironment for immobilizing glucose oxidase and the direct electrochemistry process of glucose oxidase (GOD) at Ag@C modified glassy carbon electrode (GCE) was realized. The modified electrode exhibited good response to glucose. Under optimum experimental conditions the biosensor linearly responded to glucose concentration in the range of 0.05-2.5 mM, with a detection limit of 0.02 mM (S/N = 3). The apparent Michaelis-Menten constant (K-M(app)) of the biosensor is calculated to be 1.7 mM, suggesting high enzymatic activity and affinity toward glucose. In addition, the GOD-Ag@C/Nafion/GCE shows good reproducibility and long-term stability. These results suggested that core-shell structured Ag@C is an ideal matrix for the immobilization of the redox enzymes and further the construction of the sensitive enzyme biosensor. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：579 / 587

页数：9

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