Triple-Mode Aptasensor for Sensitive and Reliable Determination of Staphylococcal Enterotoxin B

被引：10
作者：
Wu, Zhengzong [1 ]
He, Deyun [1 ]
Cui, Bo [1 ]
Jin, Zhengyu [2 ]
Xu, Enbo [3 ]
机构：
[1] Qilu Univ Technol, Shandong Acad Sci, Sch Food Sci & Engn, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
[2] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[3] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
来源：
FOOD ANALYTICAL METHODS | 2020年 / 13卷 / 06期
关键词：
Staphylococcal enterotoxin B; Surface-enhanced Raman scattering; Fluorescence spectroscopy; Circular dichroism; Rapid detection; Food analysis; IMMUNOASSAY; ASSAY;
D O I：
10.1007/s12161-020-01746-y
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products can be simultaneously detected by using surface-enhanced Raman scattering (SERS), fluorometry, and circular dichroism (CD). Under SERS mode, fluorescence mode, and CD mode, wide linear detection ranges (1-750 pg/mL, 1-750 pg/mL, and 2-500 pg center dot mL(-1)) were achieved. Furthermore, the practicality of the novel assay was evaluated by determining SEB contents in spiked milk samples. These results indicate that the triple-mode aptasensor is a potential useful tool in quantitative detection of SEB in foods.
引用
收藏
页码：1255 / 1261
页数：7
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