Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice

被引：135
作者：
Chuio, Tetsuya
Takai, Ryota
Akimoto-Tomiyama, Chiharu
Ando, Sugihiro
Minami, Eiichi
Nagamura, Yoshiaki
Kaku, Hanae
Shibuya, Naoto
Yasuda, Michiko
Nakashita, Hideo
Umemura, Kenji
Okada, Atsushi
Okada, Kazunori
Nojiri, Hideaki
Yamane, Hisakazu
机构：
[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
[2] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan
[3] Meiji Univ, Dept Life Sci, Tama Ku, Kanagawa 2160011, Japan
[4] RIKEN, Plant Acquired Immun Res Unit, Discovery Res Inst, Wako, Saitama 3510198, Japan
[5] Meiji Seika Kaisha Ltd, Kohoku Ku, Kanagawa 2228567, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2007年 / 1769卷 / 7-8期
关键词：
defense response; elicitor; Oryza sativa L. cv. Nipponbare; WRKY;
D O I：
10.1016/j.bbaexp.2007.04.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ale present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRXY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：497 / 505
页数：9
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