Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India

Background: The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as yellow mosaic virus (YMV). Hence there is need to characterize various YMV isolates associated with blackgram in Andhra Pradesh. Methods: YMV infected blackgram samples were collected from East Godavari, Kurnool and Prakasam districts of Andhra Pradesh. The Rolling Circle Amplification (RCA) based full length MYMIV DNA-A and MYMV DNA-B of three isolates were cloned and sequenced. Result: Nucleotide sequence of full length DNA-A of MYMIV-East Godavari isolate showed >96% similarity at nucleotide and >90% at amino acid level with other MYMIV isolates in NCBI database. The complete DNA-A nucleotide sequence of MYMIV-Kurnool and MYMIV- Prakasam isolates shared >99% similarity at nucleotide and >98% at amino acid level with other MYMIV isolates. The complete nucleotide sequence of MYMV DNA- B of three isolates (East Godavari, Kurnool and Prakasam) had >97% homology with other MYMV DNA-B isolates from database. The predicted amino acid sequence of MYMV DNA- B of three isolates shared >96% homology with other MYMV-B isolates. The common region (CR) sequence similarity between MYMIV- As and MYMV-Bs of East Godavari and Kurnool isolates was 76.4% and 78.3% with Prakasam isolate. The divergence between the MYMIV-A and MYMV-B of present three isolates (East Godavari, Kurnool and Prakasam) under study were ranged from 22.2 to 22.6%.