Acquisition of luteolytic capacity involves differential regulation by prostaglandin F2α of genes involved in progesterone biosynthesis in the porcine corpus luteum

被引:26
作者
Diaz, FJ [1 ]
Wiltbank, MC [1 ]
机构
[1] Univ Wisconsin, Dept Dairy Sci, Endocrinol Reprod Physiol Program, Madison, WI 53706 USA
关键词
corpus luteum; prostaglandin; porcine; luteolysis;
D O I
10.1016/j.domaniend.2004.08.002
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Luteolytic capacity is defined as the ability of corpora lutea (CL) to undergo luteolysis after prostaglandin (PG) F2alpha treatment. The mechanisms causing acquisition of luteolytic capacity are not yet identified but CL without luteolytic capacity have PGF2alpha receptors and respond to PGF2alpha. with some changes in gene expression. Inhibition of progesterone biosynthesis is a key feature of luteolysis and therefore we postulated that genes involved in progesterone biosynthesis would be regulated by PGF2alpha differently in CL with or without luteolytic capacity. Gilts on day 9 after estrus (lack luteolytic capacity) or day 17 of pseudopregnancy (with luteolytic capacity) were treated with saline or a PGF2alpha analog (cloprostenol) and CL were collected 0.5 (Experiment I) or 10 h (Experiment II) later. In Experiment III, large luteal cells from CL on day 9 or 17 were cultured for 1, 12 and 24 h with or without PGF2alpha. PGF2alpha decreased LDL receptor mRNA (27%), steroidogenic acute regulatory protein (StAR) mRNA (41%), StAR protein (75%), LH receptor mRNA (55%), and LH receptor protein (45%) at 10 h after treatment in day 17 but not day 9 CL. PGF2alpha increased DAX-1 mRNA at 0.5 h (43%) and 10 h (46%) after PGF2a in day 17 but not day 9 CL but decreased 3betaHSD mRNA (similar to20% at 10 h) in both days 9 and 17 CL. In vitro. PGF2alpha decreased StAR mRNA at 12 h only in day 17 luteal cells; however, continuous treatment with PGF2alpha for 24 h decreased StAR mRNA in both days 9 and 17 luteal cells. Thus, luteolytic capacity involves a critical change in responsiveness of DAX-1, StAR, and LH receptor to PGF2alpha that results in inhibition of luteal progesterone biosynthesis. (C) 2004 Published by Elsevier Inc.
引用
收藏
页码:172 / 189
页数:18
相关论文
共 51 条
[1]   OVARIAN FUNCTION IN PIGS FOLLOWING HYPOPHYSIAL STALK TRANSECTION OR HYPOPHYSECTOMY [J].
ANDERSON, LL ;
DYCK, GW ;
MORI, H ;
HENRICKS, DM ;
MELAMPY, RM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1967, 212 (05) :1188-&
[2]   MECHANISMS CONTROLLING CORPUS-LUTEUM FUNCTION IN SHEEP, COWS, NONHUMAN-PRIMATES, AND WOMEN ESPECIALLY IN RELATION TO THE TIME OF LUTEOLYSIS [J].
AULETTA, FJ ;
FLINT, APF .
ENDOCRINE REVIEWS, 1988, 9 (01) :88-105
[3]   In situ detection of apoptosis in regressing corpus luteum of pregnant sow: Evidence of an early presence of DNA fragmentation [J].
Bacci, ML ;
Barazzoni, AM ;
Forni, M ;
Costerbosa, GL .
DOMESTIC ANIMAL ENDOCRINOLOGY, 1996, 13 (04) :361-372
[4]   ENDOCRINE CHANGES ASSOCIATED WITH LUTEAL REGRESSION IN EWE - SECRETION OF OVARIAN ESTRADIOL, PROGESTERONE AND ANDROSTENEDIONE AND UTERINE PROSTAGLANDIN-F2-ALPHA THROUGHOUT ESTROUS-CYCLE [J].
BAIRD, DT ;
LAND, RB ;
SCARAMUZZI, RJ ;
WHEELER, AG .
JOURNAL OF ENDOCRINOLOGY, 1976, 69 (02) :275-286
[5]   LUTEINIZING-HORMONE RECEPTORS AND PROGESTERONE CONTENT IN PORCINE CORPORA-LUTEA AFTER PROSTAGLANDIN-F2-ALPHA [J].
BARB, CR ;
KRAELING, RR ;
RAMPACEK, GB ;
PINKERT, CA .
BIOLOGY OF REPRODUCTION, 1984, 31 (05) :913-919
[6]   Rat luteinizing hormone receptor messenger ribonucleic acid expression and luteolysis: Inhibition by prostaglandin F-2 alpha [J].
Bjurulf, E ;
Selstam, G .
BIOLOGY OF REPRODUCTION, 1996, 54 (06) :1350-1355
[7]   Alterations in follicular estradiol and gonadotropin receptors during development of bovine antral follicles [J].
Bodensteiner, KJ ;
Wiltbank, MC ;
Bergfelt, DR ;
Ginther, OJ .
THERIOGENOLOGY, 1996, 45 (02) :499-512
[8]   Changes in refractoriness of rabbit corpora lutea to a prostaglandin F2α analogue, alfaprostol, during pseudopregnancy [J].
Boiti, C ;
Canali, C ;
Zerani, M ;
Gobbetti, A .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1998, 56 (04) :255-264
[9]   LOSS OF LOW-DENSITY-LIPOPROTEIN UTILIZATION BY REGRESSING PORCINE LUTEAL CELLS - EFFECTS OF PROTEIN-KINASE-C ACTIVATION [J].
BRANNIAN, JD ;
CHRISTIANSON, H ;
FLYNN, S ;
KURZ, SG .
BIOLOGY OF REPRODUCTION, 1995, 52 (04) :793-797
[10]   Alteration of gonadotrophin and steroid hormone release, and of ovarian function by a GnRH antagonist in gilts [J].
Brüssow, KP ;
Schneider, F ;
Nürnberg, G .
ANIMAL REPRODUCTION SCIENCE, 2001, 66 (1-2) :117-128