A new method for setting guidelines to protect human health from agricultural exposure by using chlorpyrifos as an example

Introduction and Objectives. Guidelines set by various agencies for the control and management of chlorpyrifos cover a wide range of values reflecting difficulties in the procedures for their development. To overcome these difficulties a new method to set guidelines would be developed. Published data derived from epidemiological investigations on human populations would be used to develop a dose-response relationship for chlorpyrifos allowing the calculation of threshold values which can be used as guidelines. Materials and Method. Data from the scientific literature on human populations were collected to evaluate the adverse response doses for a range of health effects. The Cumulative Frequency Distribution (CFD) for the minimum levels of adverse effects measured in terms of the Lifetime Average Daily Dose (LADD(D)) and the Absorbed Daily Dose for neurological (ADD(DN)) and non-neurological effects were used. Results. Linear regression equations were fitted to the CFD plots giving R-2 values of 0.93 and 0.86 indicating a normal distribution of the data. Using these CFD plots, the chronic and acute threshold values were calculated at the 5% cumulative frequency level for chlorpyrifos exposure giving values at 0.5 mu g/kg/d and 3 mu g/kg/d respectively. Conclusions. Guidelines set using this technique at the values at 0.5 mu g/kg/d and 3 mu g/kg/d for chronic and acute exposure respectively provide an alternative to the currently used biological endpoint and safety factor method.