Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells

被引：216

作者：

Wang, Pengfei ^{[1
,2
]}

Rahman, Mohammad Aminur ^{[3
]}

Zhao, Zhixiang ^{[3
,4
]}

Weiss, Kristin ^{[1
,2
]}

Zhang, Chao ^{[5
]}

Chen, Zhengjia ^{[5
]}

Hurwitz, Selwyn J. ^{[6
]}

Chen, Zhuo G. ^{[3
]}

Shin, Dong M. ^{[1
,2
,3
]}

Ke, Yonggang ^{[1
,2
,7
]}

机构：

[1] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30322 USA

[2] Emory Univ, Sch Med, Atlanta, GA 30322 USA

[3] Emory Univ, Sch Med, Dept Hematol & Med Oncol, Winship Canc Inst, Atlanta, GA 30322 USA

[4] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha 410083, Hunan, Peoples R China

[5] Emory Univ, Rollins Sch Publ Hlth, Winship Canc Inst, Dept Biostat & Bioinformat Shared Resource, Atlanta, GA 30322 USA

[6] Emory Univ, Sch Med, Dept Pediat, Atlanta, GA 30322 USA

[7] Emory Univ, Dept Chem, 1515 Pierce Dr, Atlanta, GA 30322 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2018年 / 140卷 / 07期

关键词：

PLASMONIC NANOSTRUCTURES; FOLDING DNA; NANOPARTICLES; DELIVERY; MECHANISMS; TRANSPORT; POLYMERS; ENTRY;

D O I：

10.1021/jacs.7b09024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA origami is a promising molecular delivery system for a variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cancer cell lines remains elusive. We investigated the cellular uptake of four DNA-origami nanostructures (DONs) with programmed sizes and shapes in multiple human cancer cell lines. The cellular uptake efficiency of DONs was influenced by size, shape, and cell line. Scavenger receptors were responsible for the internalization of DONs into cancer cells. We observed distinct stages of the internalization process of a gold nanoparticle (AuNP)-tagged rod-shape DON, using high-resolution transmission electron microscopy. This study provides detailed understanding of cellular uptake and intracellular trafficking of DONs in cancer cells, and offers new insights for future optimization of DON-based drug delivery systems for cancer treatment.

引用

页码：2478 / 2484

页数：7

共 44 条

[1] DNA rendering of polyhedral meshes at the nanoscale [J].

Benson, Erik ;

Mohammed, Abdulmelik ;

Gardell, Johan ;

Masich, Sergej ;

Czeizler, Eugen ;

Orponen, Pekka ;

Hogberg, Bjorn .

NATURE, 2015, 523 (7561) :441-U139

[2] Mechanism for the endocytosis of spherical nucleic acid nanoparticle conjugates [J].

Choi, Chung Hang J. ;

Hao, Liangliang ;

Narayan, Suguna P. ;

Auyeung, Evelyn ;

Mirkin, Chad A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (19) :7625-7630

[3] Nanoparticle therapeutics: an emerging treatment modality for cancer [J].

Davis, Mark E. ;

Chen, Zhuo ;

Shin, Dong M. .

NATURE REVIEWS DRUG DISCOVERY, 2008, 7 (09) :771-782

[4] Folding DNA into Twisted and Curved Nanoscale Shapes [J].

Dietz, Hendrik ;

Douglas, Shawn M. ;

Shih, William M. .

SCIENCE, 2009, 325 (5941) :725-730

[5] Mechanisms of Endocytosis [J].

Doherty, Gary J. ;

McMahon, Harvey T. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2009, 78 :857-902

[6] A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads [J].

Douglas, Shawn M. ;

Bachelet, Ido ;

Church, George M. .

SCIENCE, 2012, 335 (6070) :831-834

[7] Self-assembly of DNA into nanoscale three-dimensional shapes [J].

Douglas, Shawn M. ;

Dietz, Hendrik ;

Liedl, Tim ;

Hoegberg, Bjoern ;

Graf, Franziska ;

Shih, William M. .

NATURE, 2009, 459 (7245) :414-418

[8] Observation of intracellular interactions between DNA origami and lysosomes by the fluorescence localization method [J].

Fu, Meifang ;

Dai, Luru ;

Jiang, Qiao ;

Tang, Yunqing ;

Zhang, Xiaoming ;

Ding, Baoquan ;

Li, Junbai .

CHEMICAL COMMUNICATIONS, 2016, 52 (59) :9240-9242

[9] Engineering and mapping nanocavity emission via precision placement of DNA origami [J].

Gopinath, Ashwin ;

Miyazono, Evan ;

Faraon, Andrei ;

Rothemund, Paul W. K. .

NATURE, 2016, 535 (7612) :401-+

[10] Recent insights into the biology of macrophage scavenger receptors [J].

Greaves, DR ;

Gordon, S .

JOURNAL OF LIPID RESEARCH, 2005, 46 (01) :11-20

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