Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria

被引:11
作者
Mikagi, Ayame [1 ]
Manita, Koichi [1 ]
Yoyasu, Asuka [1 ]
Tsuchido, Yuji [1 ,2 ]
Kanzawa, Nobuyuki [1 ]
Hashimoto, Takeshi [1 ]
Hayashita, Takashi [1 ]
机构
[1] Sophia Univ, Fac Sci & Technol, Dept Mat & Life Sci, Chiyoda Ku, 7-1 Kioi Cho, Tokyo 1028554, Japan
[2] Waseda Univ TWIns, Sch Adv Sci & Engn, Dept Life Sci & Med Biosci, Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan
来源
MOLECULES | 2022年 / 27卷 / 01期
关键词
bacterial recognition; dendrimer; dipicolylamine; E; coli; phenylboronic acid; S; aureus; PATHOGEN DETECTION; ESCHERICHIA-COLI; NANOPARTICLES; NANOMATERIALS; RESISTANCE;
D O I
10.3390/molecules27010256
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditopic dendrimer system had higher sensitivity and better pH tolerance than the monotopic PAMAM dendrimer probe. We also investigated the mechanisms of various ditopic PAMAM dendrimer probes and found that the selectivity toward Gram-positive bacteria was dependent on a variety of interactions. Supramolecular interactions, such as electrostatic interaction and hydrophobic interaction, per se, did not contribute to the bacterial recognition ability, nor did they improve the selectivity of the ditopic dendrimer system. In contrast, the ditopic PAMAM dendrimer probe that had a phosphate-sensing dpa group and formed a chelate with metal ions showed improved selectivity toward S. aureus. The results suggested that the targeted ditopic PAMAM dendrimer probe showed selectivity toward Gram-positive bacteria. This study is expected to contribute to the elucidation of the interaction between synthetic molecules and bacterial surface. Moreover, our novel method showed potential for the rapid and species-specific recognition of various bacteria.
引用
收藏
页数:14
相关论文
共 40 条
  • [21] Synthetic Ligand-Coated Magnetic Nanoparticles for Microfluidic Bacterial Separation from Blood
    Lee, Jung-Jae
    Jeong, Kyung Jae
    Hashimoto, Michinao
    Kwon, Albert H.
    Rwei, Alina
    Shankarappa, Sahadev A.
    Tsui, Jonathan H.
    Kohane, Daniel S.
    [J]. NANO LETTERS, 2014, 14 (01) : 1 - 5
  • [22] Quantum dot probes for bacteria distinguish Escherichia coli mutants and permit in vivo imaging
    Leevy, W. Matthew
    Lambert, Timothy N.
    Johnson, James R.
    Morris, Joshua
    Smith, Bradley D.
    [J]. CHEMICAL COMMUNICATIONS, 2008, (20) : 2331 - 2333
  • [23] Liu LH, 2009, NAT NANOTECHNOL, V4, P457, DOI [10.1038/NNANO.2009.153, 10.1038/nnano.2009.153]
  • [24] Liu Y., 2011, J MICROBIAL BIOCH TE, VS2, P2, DOI [10.4172/1948-5948.S2-001, DOI 10.4172/1948-5948.S2-001]
  • [25] Mandal P. K., 2011, American Journal of Food Technology, V6, P87
  • [26] Medved'ová A, 2012, STRUCTURE AND FUNCTION OF FOOD ENGINEERING, P71, DOI 10.5772/48175
  • [27] Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer
    Mikagi, Ayame
    Tsurufusa, Riho
    Tsuchido, Yuji
    Hashimoto, Takeshi
    Hayashita, Takashi
    [J]. SENSORS, 2021, 21 (09)
  • [28] Anion recognition and sensing with Zn(II)-dipicolylamine complexes
    Ngo, Huy Tien
    Liu, Xuejian
    Jolliffe, Katrina A.
    [J]. CHEMICAL SOCIETY REVIEWS, 2012, 41 (14) : 4928 - 4965
  • [29] Multivalency at Interfaces: Supramolecular Carbohydrate-Functionalized Graphene Derivatives for Bacterial Capture, Release, and Disinfection
    Qi, Zhenhui
    Bharate, Priya
    Lai, Chian-Hui
    Ziem, Benjamin
    Boettcher, Christoph
    Schulz, Andrea
    Beckert, Fabian
    Hatting, Benjamin
    Muelhaupt, Rolf
    Seeberger, Peter H.
    Haag, Rainer
    [J]. NANO LETTERS, 2015, 15 (09) : 6051 - 6057
  • [30] Influence of interaction between surface-modified magnetic nanoparticles with infectious biofilm components in artificial channel digging and biofilm eradication by antibiotics in vitro and in vivo
    Quan, Kecheng
    Jiang, Guimei
    Liu, Jian
    Zhang, Zexin
    Ren, Yijin
    Busscher, Henk J.
    van der Mei, Henny C.
    Peterson, Brandon W.
    [J]. NANOSCALE, 2021, 13 (08) : 4644 - 4653