Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria

被引:11
作者
Mikagi, Ayame [1 ]
Manita, Koichi [1 ]
Yoyasu, Asuka [1 ]
Tsuchido, Yuji [1 ,2 ]
Kanzawa, Nobuyuki [1 ]
Hashimoto, Takeshi [1 ]
Hayashita, Takashi [1 ]
机构
[1] Sophia Univ, Fac Sci & Technol, Dept Mat & Life Sci, Chiyoda Ku, 7-1 Kioi Cho, Tokyo 1028554, Japan
[2] Waseda Univ TWIns, Sch Adv Sci & Engn, Dept Life Sci & Med Biosci, Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan
来源
MOLECULES | 2022年 / 27卷 / 01期
关键词
bacterial recognition; dendrimer; dipicolylamine; E; coli; phenylboronic acid; S; aureus; PATHOGEN DETECTION; ESCHERICHIA-COLI; NANOPARTICLES; NANOMATERIALS; RESISTANCE;
D O I
10.3390/molecules27010256
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditopic dendrimer system had higher sensitivity and better pH tolerance than the monotopic PAMAM dendrimer probe. We also investigated the mechanisms of various ditopic PAMAM dendrimer probes and found that the selectivity toward Gram-positive bacteria was dependent on a variety of interactions. Supramolecular interactions, such as electrostatic interaction and hydrophobic interaction, per se, did not contribute to the bacterial recognition ability, nor did they improve the selectivity of the ditopic dendrimer system. In contrast, the ditopic PAMAM dendrimer probe that had a phosphate-sensing dpa group and formed a chelate with metal ions showed improved selectivity toward S. aureus. The results suggested that the targeted ditopic PAMAM dendrimer probe showed selectivity toward Gram-positive bacteria. This study is expected to contribute to the elucidation of the interaction between synthetic molecules and bacterial surface. Moreover, our novel method showed potential for the rapid and species-specific recognition of various bacteria.
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页数:14
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共 40 条
  • [11] Aggregation and Interaction of Cationic Nanoparticles on Bacterial Surfaces
    Hayden, Steven C.
    Zhao, Gengxiang
    Saha, Krishnendu
    Phillips, Ronnie L.
    Li, Xiaoning
    Miranda, Oscar R.
    Rotello, Vincent M.
    El-Sayed, Mostafa A.
    Schmidt-Krey, Ingeborg
    Bunz, Uwe H. F.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (16) : 6920 - 6923
  • [12] Bacteria-derived fluorescent carbon dots for microbial live/dead differentiation
    Hua, Xian-Wu
    Bao, Yan-Wen
    Wang, Hong-Yin
    Chen, Zhan
    Wu, Fu-Gen
    [J]. NANOSCALE, 2017, 9 (06) : 2150 - 2161
  • [13] A review of molecular recognition technologies for detection of biological threat agents
    Iqbal, SS
    Mayo, MW
    Bruno, JG
    Bronk, BV
    Batt, CA
    Chambers, JP
    [J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (11-12) : 549 - 578
  • [14] JACOBY GA, 1991, NEW ENGL J MED, V324, P601, DOI 10.1056/NEJM199102283240906
  • [15] Global trends in emerging infectious diseases
    Jones, Kate E.
    Patel, Nikkita G.
    Levy, Marc A.
    Storeygard, Adam
    Balk, Deborah
    Gittleman, John L.
    Daszak, Peter
    [J]. NATURE, 2008, 451 (7181) : 990 - U4
  • [16] Staphylococcus aureus Detection by Fluorescent Silica Nanoparticles Modified with Metal-Dipicolylamine Complexes
    Kasai, Yuna
    Kobayashi, Hiroyuki
    Tsuchido, Yuji
    Hashimoto, Takeshi
    Kanzawa, Nobuyuki
    Hayashita, Takashi
    [J]. CHEMISTRY LETTERS, 2016, 45 (07) : 749 - 751
  • [17] Selective Killing of Pathogenic Bacteria by Antimicrobial Silver Nanoparticle-Cell Wall Binding Domain Conjugates
    Kim, Domyoung
    Kwon, Seok-Joon
    Wu, Xia
    Sauve, Jessica
    Lee, Inseon
    Nam, Jahyun
    Kim, Jungbae
    Dordick, Jonathan S.
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (16) : 13317 - 13324
  • [18] Pyrophosphate selective fluorescent chemosensors based on coumarin-DPA-Cu(II) complexes
    Kim, Min Jung
    Swamy, K. M. K.
    Lee, Kyung Mi
    Jagdale, Arun R.
    Kim, Youngmee
    Kim, Sung-Jin
    Yoo, Kyung Ho
    Yoon, Juyoung
    [J]. CHEMICAL COMMUNICATIONS, 2009, (46) : 7215 - 7217
  • [19] MALDI-TOF mass spectrometry of bacteria
    Lay, JO
    [J]. MASS SPECTROMETRY REVIEWS, 2001, 20 (04) : 172 - 194
  • [20] Pathogen detection:: A perspective of traditional methods and biosensors
    Lazcka, Olivier
    Del Campo, F. Javier
    Munoz, F. Xavier
    [J]. BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) : 1205 - 1217