Astrocytes in Atp1a2-deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression

被引：6
作者：
Sugimoto, Hiroki [1 ]
Sato, Masaaki [2 ,3 ,4 ]
Nakai, Junichi [2 ,3 ,5 ]
Kawakami, Kiyoshi [1 ]
机构：
[1] Jichi Med Univ, Div Biol, Ctr Mol Med, 3311-1 Yakushiji, Shimotsuke, Tochigi 3290498, Japan
[2] Saitama Univ, Grad Sch Sci & Engn, Saitama, Japan
[3] Saitama Univ, Brain & Body Syst Sci Inst, Saitama, Japan
[4] RIKEN, Ctr Brain Sci, Lab Mental Biol, Saitama, Japan
[5] Tohoku Univ, Grad Sch Dent, Sendai, Miyagi 9808575, Japan
来源：
FEBS OPEN BIO | 2020年 / 10卷 / 06期
基金：
日本学术振兴会;
关键词：
astrocyte; Atp1a2; calcium imaging; CSD; FHM2; G-CaMP7; migraine; FAMILIAL HEMIPLEGIC MIGRAINE; CA2+ SIGNALS; CALCIUM; GLUTAMATE; NA; K-ATPASE; MECHANISMS; VASODILATION; ACTIVATION; MUTATIONS; RESPONSES;
D O I：
10.1002/2211-5463.12848
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ATP1A2 coding alpha 2 subunit of Na,K-ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2(tmCKwk/+)) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2(tmCKwk/+) crossed with transgenic mice expressing G-CaMP7 in cortical neurons and astrocytes (Atp1a2(+/-)), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2(+/-) were higher than those in wild-type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2(+/-) may contribute to FHM2 pathophysiology.
引用
收藏
页码：1031 / 1043
页数：13
相关论文
共 58 条
  • [1] [Anonymous], 2017, R LANGUAGE ENV STAT
  • [2] Benarroch EE, 2011, NEUROLOGY, V77, P281, DOI [10.1212/WNL.0b013e31822550e2, 10.1212/WNL.0b013e3182074c2f]
  • [3] Mechanisms of interleukin-1β-induced Ca2+ signals in mouse cortical astrocytes:: roles of store- and receptor-operated Ca2+ entry
    Beskina, Olga
    Miller, Anna
    Mazzocco-Spezzia, Amparo
    Pulina, Maria V.
    Golovina, Vera A.
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2007, 293 (03): : C1103 - C1111
  • [4] Monitoring activity in neural circuits with genetically encoded indicators
    Broussard, Gerard J.
    Liang, Ruqiang
    Tian, Lin
    [J]. FRONTIERS IN MOLECULAR NEUROSCIENCE, 2014, 7
  • [5] Defective glutamate and K+ clearance by cortical astrocytes in familial hemiplegic migraine type 2
    Capuani, Clizia
    Melone, Marcello
    Tottene, Angelita
    Bragina, Luca
    Crivellaro, Giovanna
    Santello, Mirko
    Casari, Giorgio
    Conti, Fiorenzo
    Pietrobon, Daniela
    [J]. EMBO MOLECULAR MEDICINE, 2016, 8 (08) : 967 - 986
  • [6] Haploinsufficiency of ATP1A2 encoding the Na+/K+ pump α2 subunit associated with familial hemiplegic migraine type 2
    De Fusco, M
    Marconi, R
    Silvestri, L
    Atorino, L
    Rampoldi, L
    Morgante, L
    Ballabio, A
    Aridon, P
    Casari, G
    [J]. NATURE GENETICS, 2003, 33 (02) : 192 - 196
  • [7] Epilepsy as part of the phenotype associated with ATP1A2 mutations
    Deprez, Liesbet
    Weckhuysen, Sarah
    Peeters, Katelijne
    Deconinck, Tine
    Claeys, Kristl G.
    Claes, Lieve R. F.
    Suls, Arvid
    Van Dyck, Tine
    Palmini, Andre
    Matthijs, Gert
    Van Paesschen, Wim
    De Jonghe, Peter
    [J]. EPILEPSIA, 2008, 49 (03) : 500 - 508
  • [8] Mutation in the neuronal voltage-gated sodium channel SCN1A in familial hemiplegic migraine
    Dichgans, M
    Freilinger, T
    Eckstein, G
    Babini, E
    Lorenz-Depiereux, B
    Biskup, S
    Ferrari, MD
    Herzog, J
    van den Maagdenberg, AMJM
    Pusch, M
    Strom, TM
    [J]. LANCET, 2005, 366 (9483) : 371 - 377
  • [9] Dynamics of Ionic Shifts in Cortical Spreading Depression
    Enger, Rune
    Tang, Wannan
    Vindedal, Gry Fluge
    Jensen, Vidar
    Helm, P. Johannes
    Sprengel, Rolf
    Looger, Loren L.
    Nagelhus, Erlend A.
    [J]. CEREBRAL CORTEX, 2015, 25 (11) : 4469 - 4476
  • [10] Migraine pathophysiology: lessons from mouse models and human genetics
    Ferrari, Michel D.
    Klever, Roselin R.
    Terwindt, Gisela M.
    Ayata, Cenk
    van den Maagdenberg, Arn M. J. M.
    [J]. LANCET NEUROLOGY, 2015, 14 (01) : 65 - 80
123456