Downregulation of miR-27a-3p may increase hematoma volume in rats with intracerebral hemorrhage

Objective: The aim of this study was to investigate expression and mechanisms of action of miR-27a-3p in hematoma tissue of rats with intracerebral hemorrhage (ICH). Methods: Intracerebral injections of 2 mu l of bacterial collagen were applied to establish rat models of ICH in control and experimental groups. In the experimental group, miR-27a-3p inhibitors were injected into the right ventricle of rats before initiation of the model. RT-PCR was conducted to measure relative expression of miR-27a-3p in the hematoma of ICH rats. Results: Relative expression of miR-27a-3p in brain hematoma tissue in the experimental group was significantly lower than the control group on days 1 and 3 (t = 21.140, P < 0.001 on day 1; t = 12.480, P < 0.001 on day 3). The volume of hematoma in rats of the experimental group was significantly greater than the control group on day 1 (t = 13.110, P < 0.001). miR-27a-3p inhibitors significantly downregulated miR-27a-3p (t = 5.061, P < 0.001). In rats with ICH, downregulation of miR-27a-3p increased hematoma volume (t = 7.453, P < 0.001) and aggravated neurological deficits (t = 7.992, P < 0.001). Conclusion: miR-27a-3p may be involved in the formation and development of cerebral hematoma after ICH. Therefore, miR-27a-3p could become a biological therapeutic target for ICH.