Modification of the embryogenic response of Coffea arabica by the nitrogen source

被引：16
作者：
Fuentes-Cerda, CFJ
Monforte-González, M
Méndez-Zeel, M
Rojas-Herrera, R
Loyola-Vargas, VM
机构：
[1] Ctr Invest Cient Yucatan, Merida 97200, Yucatan, Mexico
[2] Inst Nacl Ciencias Agr, La Habana, Cuba
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期
关键词：
Coffea spp; direct embryogenesis; nitrogen source; somatic embryogenesis;
D O I：
10.1023/A:1010545818671
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Somatic embryogenesis was induced in coffee from in vitro cultured plants as starting material and the faster response obtained allowed lines from selected plants to be generated more quickly. In contrast to other systems, where embryos take 2 or 3 months to develop, globular embryos were obtained after 3 weeks. The optimum nitrogen concentrations for embryogenesis were between 3.75 and 15 mM nitrogen with a nitrate/ammonium molar ratio of 2:1 or 1:2.
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页码：1341 / 1343
页数：3
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