Modification of the embryogenic response of Coffea arabica by the nitrogen source

被引：16

作者：

Fuentes-Cerda, CFJ

Monforte-González, M

Méndez-Zeel, M

Rojas-Herrera, R

Loyola-Vargas, VM

机构：

[1] Ctr Invest Cient Yucatan, Merida 97200, Yucatan, Mexico

[2] Inst Nacl Ciencias Agr, La Habana, Cuba

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期

关键词：

Coffea spp; direct embryogenesis; nitrogen source; somatic embryogenesis;

D O I：

10.1023/A:1010545818671

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Somatic embryogenesis was induced in coffee from in vitro cultured plants as starting material and the faster response obtained allowed lines from selected plants to be generated more quickly. In contrast to other systems, where embryos take 2 or 3 months to develop, globular embryos were obtained after 3 weeks. The optimum nitrogen concentrations for embryogenesis were between 3.75 and 15 mM nitrogen with a nitrate/ammonium molar ratio of 2:1 or 1:2.

引用

收藏

页码：1341 / 1343

页数：3

相关论文

共 10 条

[1] ENHANCED SOMATIC EMBRYO PRODUCTION BY CONDITIONED MEDIA IN CELL-SUSPENSION CULTURES OF DAUCUS-CAROTA [J].

CHUNG, WJ ;

PEDERSEN, H ;

CHIN, CK .

BIOTECHNOLOGY LETTERS, 1992, 14 (09) :837-840

[2] THE INORGANIC NO3--NH4+ RATIO INFLUENCES PLANT-REGENERATION AND AUXIN SENSITIVITY IN PRIMARY CALLUS DERIVED FROM IMMATURE EMBRYOS OF INDICA RICE (ORYZA-SATIVA L) [J].

GRIMES, HD ;

HODGES, TK .

JOURNAL OF PLANT PHYSIOLOGY, 1990, 136 (03) :362-367

[3] AMMONIUM REQUIREMENT FOR EMBRYOGENESIS IN VITRO [J].

HALPERIN, W ;

WETHEREL.DF .

NATURE, 1965, 205 (4970) :519-&

[4] Stage-specific nitrogen metabolism in developing carrot somatic embryos [J].

Joy, RW ;

McIntyre, DD ;

Vogel, HJ ;

Thorpe, TA .

PHYSIOLOGIA PLANTARUM, 1996, 97 (01) :149-159

[5] ROLE OF EXOGENOUS REDUCED NITROGEN AND SUCROSE IN RAPID HIGH-FREQUENCY SOMATIC EMBRYOGENESIS IN MEDICAGO-SATIVA [J].

MEIJER, EGM ;

BROWN, DCW .

PLANT CELL TISSUE AND ORGAN CULTURE, 1987, 10 (01) :11-19

[6] EMBRYOGENESIS AND DEVELOPMENT OF ISOLATED BARLEY (HORDEUM-VULGARE L) MICROSPORES ARE INFLUENCED BY THE AMOUNT AND COMPOSITION OF NITROGEN-SOURCES IN CULTURE MEDIA [J].

MORDHORST, AP ;

LORZ, H .

JOURNAL OF PLANT PHYSIOLOGY, 1993, 142 (04) :485-492

[7] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[8] NITROGEN COMPOUNDS AS FACTORS OF EMBRYOGENESIS IN VITRO [J].

REINERT, J ;

TAZAWA, M ;

SEMENOFF, S .

NATURE, 1967, 216 (5121) :1215-&

[9] SOURCES OF NITROGEN SUPPORTING GROWTH AND EMBRYOGENESIS IN CULTURED WILD CARROT TISSUE [J].

WETHERELL, DF ;

DOUGALL, DK .

PHYSIOLOGIA PLANTARUM, 1976, 37 (02) :97-103

[10] EMBRYOGENIC CALLUS INDUCTION FROM COFFEA-ARABICA LEAF EXPLANTS BY BENZYLADENINE [J].

YASUDA, T ;

FUJII, Y ;

YAMAGUCHI, T .

PLANT AND CELL PHYSIOLOGY, 1985, 26 (03) :595-597