A novel viral responsive protein (MjVRP) from Marsupenaeus japonicus haemocytes is involved in white spot syndrome virus infection

被引：10
|
作者：
Elbahnaswy, Samia [1 ,2 ]
Koiwai, Keiichiro [1 ]
Zaki, Viola H. [2 ]
Shaheen, Adel A. [3 ]
Kondo, Hidehiro [1 ]
Hirono, Iktio [1 ]
机构：
[1] Tokyo Univ Marine Sci & Technol, Lab Genome Sci, Minato Ku, Konan 4-5-7, Tokyo 1088477, Japan
[2] Mansoura Univ, Dept Internal Med Infect & Fish Dis, Fac Vet Med, Mansoura 35516, Egypt
[3] Benha Univ, Dept Fish Dis & Management, Fac Vet Med, Banha 13518, Egypt
来源：
FISH & SHELLFISH IMMUNOLOGY | 2017年 / 70卷
基金：
日本科学技术振兴机构;
关键词：
MjVRP; Marsupenaeus japonicus; Haemocytes; White spot syndrome virus; Flow cytometry; Immunofluorescence; BLACK TIGER SHRIMP; FRESH-WATER CRAYFISH; PENAEUS-MONODON; KURUMA SHRIMP; IMMUNE-RESPONSES; SYSTEM; CRUSTACEAN; WSSV; COMPONENTS; MEMBRANE;
D O I：
10.1016/j.fsi.2017.09.045
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A viral responsive protein (MjVRP) was characterized from Marsupenaeus japonicus haemocytes. In amino acid homology and phylogenetic tree analyses, MjVRP clustered in the same group with the viral responsive protein of Penaeus monodon (PmVRP15), showing 34% identity. MjVRP transcripts were mainly expressed in haemocytes and the lymphoid organ. Western blotting likewise showed that MjVRP was strongly expressed in haemocytes and the lymphoid organ. Immunostaining detected MjVRP within the cytosol next to the perinuclear region in some haemocytes. Experimental challenge with white spot syndrome virus (WSSV) significantly up-regulated the mRNA level of MjVRP in the M. japonicus haemocytes at 6 and 48 h. Flow cytometry and indirect immunofluorescence assays revealed that the ratio of MjVRP(+) haemocytes significantly increased 24 and 48 h post-WSSV infection. These results suggest that MjVRP(+) haemocytes have a supporting role in the pathogenesis of WSSV. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：638 / 647
页数：10
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