3D second harmonic generation imaging tomography by multi-view excitation

被引：15

作者：

Campbell, Kirby R. ^{[1
]}

Wen, Bruce ^{[1
,2
]}

Shelton, Emily M. ^{[1
]}

Swader, Robert ^{[2
]}

Cox, Benjamin L. ^{[1
,2
]}

Eliceiri, Kevin ^{[1
,2
]}

Campagnola, Paul J. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biomed Engn, Lab Opt & Computat Instrumentat, 1550 Engn Dr, Madison, WI 53706 USA

[2] Morgridge Inst Res, 330 N Orchard St, Madison, WI 53715 USA

来源：

OPTICA | 2017年 / 4卷 / 10期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

PLANE ILLUMINATION MICROSCOPY; NONLINEAR MICROSCOPY; TAIL TENDON; COLLAGEN; REGISTRATION;

D O I：

10.1364/OPTICA.4.001171

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Biological tissues have complex 3D collagen fiber architecture that cannot be fully visualized by conventional second harmonic generation (SHG) microscopy due to electric dipole considerations. We have developed a multi-view SHG imaging platform that successfully visualizes all orientations of collagen fibers. This is achieved by rotating tissues relative to the excitation laser plane of incidence, where the complete fibrillar structure is then visualized following registration and reconstruction. We evaluated high frequency and Gaussian weighted fusion reconstruction algorithms, and found the former approach performs better in terms of the resulting resolution. The new approach is a first step toward SHG tomography. (C) 2017 Optical Society of America

引用

页码：1171 / 1179

页数：9

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