Grapevine Green Pruning Residues as a Promising and Sustainable Source of Bioactive Phenolic Compounds

被引:19
作者
Acquadro, Stefano [1 ]
Appleton, Silvia [1 ]
Marengo, Arianna [1 ]
Bicchi, Carlo [1 ]
Sgorbini, Barbara [1 ]
Mandrone, Manuela [2 ]
Gai, Francesco [3 ]
Peiretti, Pier Giorgio [3 ]
Cagliero, Cecilia [1 ]
Rubiolo, Patrizia [1 ]
机构
[1] Univ Torino, Dipartimento Sci & Tecnol Farmaco, Via Pietro Giuria 9, I-10125 Turin, Italy
[2] Alma Mater Studiorum Univ Bologna, Dipartimento Farm & Biotecnol, Via Irnerio 42, I-40126 Bologna, Italy
[3] CNR, Ist Sci & Prod Alimentari, Lgo Paolo Braccini 2, I-10095 Grugliasco, TO, Italy
关键词
Vitis vinifera L; green pruning residues; viticulture sustainability; phenolic pattern; antioxidant activity; HPLC-PDA-ESI-MS/MS; colorimetric in vitro assays; VITIS-VINIFERA L; ULTRASOUND-ASSISTED EXTRACTION; ELLAGIC ACID-DERIVATIVES; WINERY BY-PRODUCTS; IN-VITRO; ANTIOXIDANT; LEAVES; RECOVERY; FOOD; FLAVONOLS;
D O I
10.3390/molecules25030464
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Green pruning residues (GPRs) and leaves from 16 red and white Vitis vinifera L. cultivars from Piedmont (Italy) were studied. The investigated samples were extracted by ultrasound-assisted extraction optimized by an experimental design, and quali- and quantitatively analyzed by HPLC-PDA-MS/MS. GPRs and leaves show a similar polyphenolic pattern, with quercetin 3-O-glucuronide, caftaric acid, and quercetin 3-O-glucoside as the main components, although in variable proportions. The HPLC results were related to the antioxidant activity, measured as total phenolic content and through DPPH and ABTS assays with similar results. Colorimetric in vitro assays, offline combined with HPLC-PDA analysis, determine which compounds contribute to the antioxidant activity in terms of radical scavenging abilities. Valorization of GPRs is a potential source of natural compounds that could be of interest in the health field, increasing their economic value together with a positive effect on the environment.
引用
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页数:17
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