Release of infectious virus and cytokines in nasopharyngeal swabs from individuals infected with non-alpha or alpha SARS-CoV-2 variants: an observational retrospective study

被引：14

作者：

Monel, Blandine ^{[1
,2
]}

Planas, Delphine ^{[1
,2
,3
]}

Grzelak, Ludivine ^{[1
,2
,4
]}

Smith, Nikaia ^{[5
]}

Robillard, Nicolas ^{[6
]}

Staropoli, Isabelle ^{[1
,2
]}

Goncalves, Pedro ^{[7
,8
]}

Porrot, Francoise ^{[1
,2
]}

Guivel-Benhassine, Florence ^{[1
,2
]}

Guinet, Nathalie Demory ^{[9
]}

Rodary, Julien ^{[6
]}

Puech, Julien ^{[6
]}

Euzen, Victor ^{[6
]}

Belec, Laurent ^{[6
,10
]}

Orvoen, Galdric ^{[11
]}

Nunes, Lea ^{[12
]}

Moulin, Veronique ^{[12
]}

Fourgeaud, Jacques ^{[13
,14
]}

Wack, Maxime ^{[15
]}

Imbeaud, Sandrine ^{[16
,17
]}

Campagne, Pascal ^{[18
]}

Duffy, Darragh ^{[5
]}

Di Santo, James P. ^{[7
,8
]}

Bruel, Timothee ^{[1
,2
,3
]}

Pere, Helene ^{[16
,17
]}

Veyer, David ^{[6
,16
,17
]}

Schwartz, Olivier ^{[1
,2
,3
]}

机构：

[1] Inst Pasteur, Dept Virol, Virus & Immun Unit, Paris, France

[2] CNRS UMR 3569, Paris, France

[3] Vaccine Res Inst, Creteil, France

[4] Univ Paris, Sorbonne Paris Cite, Paris, France

[5] Inst Pasteur, Dept Immunol, Translat Immunol Lab, Inserm U1223, Paris, France

[6] Hop Europeen Georges Pompidou, Lab Virol, Serv Microbiol, Paris, France

[7] Inst Pasteur, Inserm U1223, Innate Immun Unit, Dept Immunol, Paris, France

[8] Inserm U1223, Paris, France

[9] Hop Europeen Georges Pompidou, Serv Med Travail, Paris, France

[10] Univ Paris, Fac Med, PARCC, Hop Europeen Georges Pompidou,INSERM U970, Paris, France

[11] Hop Vaugirard, AP HP, Serv Geriatrie, Paris, France

[12] Hop Corentin Celton, AP HP, Serv Geriatrie, Issy Les Moulineaux, France

[13] Univ Paris, Inst Imagine, EHU 7328 PACT, Paris, France

[14] Necker Enfants Malad Hosp, AP HP, Virol Dept, Paris, France

[15] Hop Europeen Georges Pompidou, Dept Informat Med Biostat & Sante Publ, Paris, France

[16] Univ Paris, Ctr Rech Cordeliers, Funct Genom Solid Tumors FunGeST, INSERM, Paris, France

[17] Sorbonne Univ, Paris, France

[18] Inst Pasteur, Bioinformat & Biostat Hub, Paris, France

来源：

EBIOMEDICINE | 2021年 / 73卷

关键词：

COVID-19; SARS-CoV-2; Nasal swabs; variants; infectious particles; VIRAL LOAD; COVID-19;

D O I：

10.1016/j.ebiom.2021.103637

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: The dynamics of SARS-CoV-2 alpha variant shedding and immune responses at the nasal mucosa remain poorly characterised. Methods: We measured infectious viral release, antibodies and cytokines in 426 PCR+ nasopharyngeal swabs from individuals harboring non-alpha or alpha variants. Findings: With both lineages, viral titers were variable, ranging from 0 to >10(6) infectious units. Rapid antigenic diagnostic tests were positive in 94% of samples with infectious virus. 68 % of individuals carried infectious virus within two days after onset of symptoms. This proportion decreased overtime. Viable virus was detected up to 14 days. Samples containing anti-spike IgG or IgA did not generally harbor infectious virus. Ct values were slightly but not significantly lower with alpha. This variant was characterized by a fast decrease of infectivity overtime and a marked release of 13 cytokines (including IFN-b, IP-10 and IL-10). Interpretation: The alpha variant displays modified viral decay and cytokine profiles at the nasopharyngeal mucosae during symptomatic infection. (C) 2021 The Authors. Published by Elsevier B.V.

引用

页数：12

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