Size-Dependant Cellular Uptake of Dendritic Polyglycerol

被引：53

作者：

Reichert, Stephanie ^{[1
]}

Welker, Pia ^{[2
]}

Calderon, Marcelo ^{[1
]}

Khandare, Jayant ^{[1
]}

Mangoldt, Dorothea ^{[2
]}

Licha, Kai ^{[2
]}

Kainthan, Rajesh K. ^{[3
]}

Brooks, Donald E. ^{[3
]}

Haag, Rainer ^{[1
]}

机构：

[1] Free Univ Berlin, Dept Biol Chem & Pharm, D-14195 Berlin, Germany

[2] Mivenion GmbH, D-10115 Berlin, Germany

[3] Univ British Columbia, Ctr Blood Res, Dept Pathol & Lab Med, Vancouver, BC V6T 2B5, Canada

来源：

SMALL | 2011年 / 7卷 / 06期

基金：

加拿大健康研究院;

关键词：

MACROMOLECULAR THERAPEUTICS; HYPERBRANCHED POLYGLYCEROLS; DESIGNING DENDRIMERS; DRUG-DELIVERY; IN-VIVO; TRANSPORT; NANOPARTICLES; PERMEABILITY; NANODEVICES; ENDOCYTOSIS;

D O I：

10.1002/smll.201002220

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To study the mechanism of cellular internalization, hyperbranched polyether derivatives consisting of amino-bearing hyperbranched polyglycerols (HPGs) of varied molecular mass and size range are designed and synthesized. HPGs were further fluorescently labelled by conjugating maleimido indocarbocyanine dye (ICC-mal). The conjugates are characterized by UV-vis spectroscopy, fluorescence profile, zeta potential, and dynamic light scattering. The uptake mechanism is studied by fluorescence-activated cell sorting (FACS) analysis, fluorescence spectroscopy, and confocal microscopy with human lung cancer cells A549, human epidermoid carcinoma cells A431, and human umbilical vein endothelial cells (HUVEC) cells. For the first time, the results suggest that the higher-molecular-weight HPGs (40-870 kDa) predominantly accumulate in the cytoplasm much better than their low-molecular-weight counterparts (2-20 kDa). The HPG nanocarriers discussed here have many biomedical implications, particularly for delivering drugs to the targeted site.

引用

页码：820 / 829

页数：10

共 41 条

[1] Self-immolative dendrimers [J].

Amir, RJ ;

Pessah, N ;

Shamis, M ;

Shabat, D .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (37) :4494-4499

[2]

BAUMGARTNER W, 2007, NANOTECHNOLOGIE MED

[3] Development of efficient acid cleavable multifunctional prodrugs derived from dendritic polyglycerol with a poly(ethylene glycol) shell [J].

Calderon, Marcelo ;

Welker, Pia ;

Licha, Kai ;

Fichtner, Iduna ;

Graeser, Ralph ;

Haag, Rainer ;

Kratz, Felix .

JOURNAL OF CONTROLLED RELEASE, 2011, 151 (03) :295-301

[4] Dendritic Polyglycerols for Biomedical Applications [J].

Calderon, Marcelo ;

Quadir, Mohiuddin Abdul ;

Sharma, Sunil Kumar ;

Haag, Rainer .

ADVANCED MATERIALS, 2010, 22 (02) :190-218

[5] Development of enzymatically cleavable prodrugs derived from dendritic polyglycerol [J].

Calderon, Marcelo ;

Graeser, Ralph ;

Kratz, Felix ;

Haag, Rainer .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2009, 19 (14) :3725-3728

[6] Dendritic polyglycerol sulfates as multivalent inhibitors of inflammation [J].

Dernedde, Jens ;

Rausch, Alexandra ;

Weinhart, Marie ;

Enders, Sven ;

Tauber, Rudolf ;

Licha, Kai ;

Schirner, Michael ;

Zuegel, Ulrich ;

von Bonin, Arne ;

Haag, Rainer .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (46) :19679-19684

[7] INHIBITION OF ENDOCYTOSIS BY ANTI-CLATHRIN ANTIBODIES [J].

DOXSEY, SJ ;

BRODSKY, FM ;

BLANK, GS ;

HELENIUS, A .

CELL, 1987, 50 (03) :453-463

[8] Hyperbranched polymers: from synthesis to applications [J].

Gao, C ;

Yan, D .

PROGRESS IN POLYMER SCIENCE, 2004, 29 (03) :183-275

[9] 60 YEARS OF STAUDINGER REACTION [J].

GOLOLOBOV, YG ;

ZHMUROVA, IN ;

KASUKHIN, LF .

TETRAHEDRON, 1981, 37 (03) :437-472

[10]

HAAG R, 2002, MOL BIOTECHNOL, V90, P257

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