Coralloluteibacterium thermophilus sp nov., A Gammaproteobacterium Isolated from an Oil Reservoir

A Gram-negative, yellow-pigmented, aerobic bacterium, designated strain B51-30(T), was isolated from oil-well production liquid in Baolige oilfield, China. The strain was able to grow at pH 6-10 (optimum at pH 7.5), in 0-6% (w/v) NaCl (optimum at 1%, w/v) at 15-55 degrees C (optimum at 45 degrees C). Cells of the isolate were non-motile and non-spore-forming rods. The major cellular fatty acids were iso-C-15:0, iso-C-11:0, iso-C-11:0 3OH, iso-C-17:1 9c, and iso-C-17:0. Ubiquinone 8 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylethanolamine and diphosphatidylglycerol. The genomic DNA G+C content of the isolate was 70.6mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B51-30(T) was most closely related to Coralloluteibacterium stylophorae KCTC 52167(T) (98.7% similarity). The two strains showed DNA-DNA relatedness values of 58.5%. Genotypic and phenotypic features indicate that strain B51-30(T) represents a novel species of the genus Coralloluteibacterium, for which the name Coralloluteibacterium thermophilus sp. nov. is proposed. The type strain is B51-30(T) (=CGMCC 1.13574(T)=KCTC 62780(T)).