NeuO: a Fluorescent Chemical Probe for Live Neuron Labeling

被引：69

作者：

Er, Jun Cheng ^{[1
]}

Leong, Cheryl ^{[2
]}

Teoh, Chai Lean ^{[2
]}

Yuan, Qiang ^{[3
]}

Merchant, Paolomi ^{[4
]}

Dunn, Matthew ^{[4
]}

Sulzer, David ^{[5
,6
]}

Sames, Dalibor ^{[4
]}

Bhinge, Akshay ^{[7
]}

Kim, Dongyoon ^{[8
]}

Kim, Seong-Min ^{[8
]}

Yoon, Myung-Han ^{[8
]}

Stanton, Lawrence W. ^{[7
]}

Je, Shawn H. ^{[3
]}

Yun, Seong-Wook ^{[2
]}

Chang, Young-Tae ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Agcy Sci Technol & Res, Singapore BioImaging Consortium, Singapore 138667, Singapore

[3] Natl Univ Singapore, Duke NUS Grad Med Sch, Singapore 169857, Singapore

[4] Columbia Univ, Dept Chem, New York, NY 10027 USA

[5] Columbia Univ, Dept Neurol, New York, NY 10032 USA

[6] Columbia Univ, Dept Psychiat, New York, NY 10032 USA

[7] Agcy Sci Technol & Res, Genome Inst Singapore, Singapore 138672, Singapore

[8] Gwangju Inst Sci & Technol, Kwangju 500712, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 08期

关键词：

BODIPY; fluorescent probes; imaging agents; live neurons; MICROGLIA;

D O I：

10.1002/anie.201408614

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation invivo and invitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.

引用

页码：2442 / 2446

页数：5

共 18 条

[1] NEuronMOrphological analysis tool: open-source software for quantitative morphometrics [J].

Billeci, Lucia ;

Magliaro, Chiara ;

Pioggia, Giovanni ;

Ahluwalia, Arti .

FRONTIERS IN NEUROINFORMATICS, 2013, 7

[2] Isolation and culture of adult rat hippocampal neurons [J].

Brewer, GJ .

JOURNAL OF NEUROSCIENCE METHODS, 1997, 71 (02) :143-155

[3] Role of histone deacetylase activity in the developing lateral line neuromast of zebrafish larvae [J].

He, Yingzi ;

Mei, Honglin ;

Yu, Huiqian ;

Sun, Shan ;

Ni, Wenli ;

Li, Huawei .

EXPERIMENTAL AND MOLECULAR MEDICINE, 2014, 46 :e94-e94

[4] DIL AND DIO - VERSATILE FLUORESCENT DYES FOR NEURONAL LABELING AND PATHWAY TRACING [J].

HONIG, MG ;

HUME, RI .

TRENDS IN NEUROSCIENCES, 1989, 12 (09) :333-&

[5] A simplified method for isolating highly purified neurons, oligodendrocytes, astrocytes, and microglia from the same human fetal brain tissue [J].

Jana, Malabendu ;

Jana, Arundhati ;

Pal, Utpal ;

Pahan, Kalipada .

NEUROCHEMICAL RESEARCH, 2007, 32 (12) :2015-2022

[6] Improved method for combination of immunocytochemistry and Nissl staining [J].

Kadar, Andrea ;

Wittmann, Gabor ;

Liposits, Zsolt ;

Fekete, Csaba .

JOURNAL OF NEUROSCIENCE METHODS, 2009, 184 (01) :115-118

[7] Microglia specific fluorescent probes for live cell imaging [J].

Leong, Cheryl ;

Lee, Sung Chan ;

Ock, Jiyeon ;

Li, Xin ;

See, Peter ;

Park, Sung Jin ;

Ginhoux, Florent ;

Yun, Seong-Wook ;

Chang, Young-Tae .

CHEMICAL COMMUNICATIONS, 2014, 50 (09) :1089-1091

[8]

Maecker H., 2008, Nat. Methods, V5

[9] Two-photon imaging in living brain slices [J].

Mainen, ZF ;

Maletic-Savatic, M ;

Shi, SH ;

Hayashi, Y ;

Malinow, R ;

Svoboda, K .

METHODS, 1999, 18 (02) :231-+

[10] Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo [J].

Nimmerjahn, A ;

Kirchhoff, F ;

Kerr, JND ;

Helmchen, F .

NATURE METHODS, 2004, 1 (01) :31-37

← 1 2 →