Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana

被引：12

作者：

Valenzuela-Miranda, Diego ^{[1
]}

Gallardo-Escarate, Cristian ^{[1
]}

Valenzuela-Munoz, Valentina ^{[1
]}

Farlora, Rodolfo ^{[1
]}

Gajardo, Gonzalo ^{[2
]}

机构：

[1] Univ Concepcion, Interdisciplinary Ctr Aquaculture Res INCAR, Lab Biotechnol & Aquat Genom, Concepcion, Chile

[2] Univ Los Lagos, Lab Genet Acuicultura & Biodiveisidad, Osorno 1305, Chile

来源：

MARINE GENOMICS | 2014年 / 18卷

关键词：

RNA-Seq; SNP; Brine shrimp; Artemia Transcriptome;

D O I：

10.1016/j.margen.2014.10.007

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to enhance genomic resources for the brine shrimp Artemia franciscana, RNA-Seq analysis was conducted for adult females and males. Through de novo assembly, 36,896 high quality contigs were obtained, of which 13,749 sequences were annotated with arthropod sequences. Just 4.5% matched against previously reported sequences for Artemia spp. Additionally, different transcriptional patterns between males and females were found, evidencing sex-related transcriptional responses. Furthermore, 221 and 534 putative SNPs were identified exclusively in males and females, respectively. These results will build the foundation for further genomic studies in A. franciscana. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：151 / 154

页数：4

共 9 条

[1] Blast2GO:: a universal tool for annotation, visualization and analysis in functional genomics research [J].