Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

被引：129

作者：

Li, Zhen ^{[1
,2
]}

Sun, Liang ^{[3
]}

Zhang, Yufei ^{[1
,2
]}

Dove, Andrew P. ^{[3
]}

O'Reilly, Rachel K. ^{[3
]}

Chen, Guosong ^{[1
,2
]}

机构：

[1] Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China

[2] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China

[3] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

来源：

ACS MACRO LETTERS | 2016年 / 5卷 / 09期

基金：

中国国家自然科学基金; 英国工程与自然科学研究理事会;

关键词：

CYLINDRICAL MICELLES; GOLD NANOPARTICLES; MULTIVALENT LIGAND; SIZE; BINDING;

D O I：

10.1021/acsmacrolett.6b00419

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The shells of various poly(DL-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(L-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (L-n X W-n = 215 X 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (L-n X W-n = 99X 50 nm) and spherical GNPs (D-n = 46 nm).

引用

页码：1059 / 1064

页数：6

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