Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

被引：129
作者：
Li, Zhen [1 ,2 ]
Sun, Liang [3 ]
Zhang, Yufei [1 ,2 ]
Dove, Andrew P. [3 ]
O'Reilly, Rachel K. [3 ]
Chen, Guosong [1 ,2 ]
机构：
[1] Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[2] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
[3] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England
来源：
ACS MACRO LETTERS | 2016年 / 5卷 / 09期
基金：
中国国家自然科学基金; 英国工程与自然科学研究理事会;
关键词：
CYLINDRICAL MICELLES; GOLD NANOPARTICLES; MULTIVALENT LIGAND; SIZE; BINDING;
D O I：
10.1021/acsmacrolett.6b00419
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The shells of various poly(DL-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(L-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (L-n X W-n = 215 X 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (L-n X W-n = 99X 50 nm) and spherical GNPs (D-n = 46 nm).
引用
收藏
页码：1059 / 1064
页数：6
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