Isolation and characterization of microsatellite loci for the endangered Midwife Betic toad Alytes dickhilleni (Discoglossidae)

被引:2
|
作者
Albert, Eva M. [1 ]
Arroyo, Juan M. [1 ]
Godoy, Jose A. [1 ]
机构
[1] CSIC, Estn Biol Donana, Dept Integrat Biol, Seville 41092, Spain
关键词
Alytes dickhilleni; Amphibian decline; Iberian Peninsula; SSR;
D O I
10.1007/s12686-010-9334-y
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
We have developed eleven polymorphic microsatellite loci for the amphibian Alytes dickhilleni using an enriched-library approach. We detected 98 alleles in 50 individuals genotyped (mean number of alleles per locus was 8.91) in two different populations in South East Spain. Expected heterozygosities ranged from 0.324 to 0.891 in one population (Arroyo Guadahornillos) and 0.424-0.909 in the other population (Cueva Paria). The levels of polymorphism of the developed markers render them readily applicable for population genetic studies of diversity, structure, and migration.
引用
收藏
页码:251 / 253
页数:3
相关论文
共 50 条
  • [21] Intraspecific genetic variation in the common midwife toad (Alytes obstetricans): subspecies assignment using mitochondrial and microsatellite markers
    Maia-Carvalho, Bruno
    Goncalves, Helena
    Martinez-Solano, Inigo
    Gutierrez-Rodriguez, Jorge
    Lopes, Susana
    Ferrand, Nuno
    Sequeira, Fernando
    JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, 2014, 52 (02) : 170 - 175
  • [22] Isolation and characterization of microsatellite loci in the endangered freshwater fish Varicorhinus alticorpus (Cyprinidae)
    Chiang, Tzen-Yuh
    Lee, Teh-Wang
    Lin, Feng-Jiau
    Huang, Kuo-Hua
    Lin, Hung-Du
    CONSERVATION GENETICS, 2008, 9 (05) : 1399 - 1401
  • [23] ISOLATION AND CHARACTERIZATION OF 15 MICROSATELLITE LOCI IN FOUR ENDANGERED AMENTOTAXUS SPECIES (TAXACEAE)
    Ho, Chin-Shang
    Chen, Chaur-Tzuhn
    Ko, Ya-Zhu
    Ge, Xue-Jun
    Hung, Kuo-Hsiang
    Hsu, Tsai-Wen
    Chiang, Yu-Chung
    AMERICAN JOURNAL OF BOTANY, 2012, 99 (04) : E157 - E159
  • [24] Isolation and characterization of microsatellite loci in the globally endangered Corncrake, Crex crex Linne
    Gautschi, B
    Arter, MK
    Husi, RE
    Wettstein, W
    Schmid, B
    CONSERVATION GENETICS, 2002, 3 (04) : 451 - 453
  • [25] Characterization and isolation of microsatellite loci from the endangered North Atlantic right whale
    Waldick, RC
    Brown, MW
    White, BN
    MOLECULAR ECOLOGY, 1999, 8 (10) : 1763 - 1765
  • [26] Isolation and characterization of microsatellite loci in the globally endangered Corncrake, Crex crex Linné
    Barbara Gautschi
    Marianne Klug Arter
    René Husi
    Walter Wettstein
    Bernhard Schmid
    Conservation Genetics, 2002, 3 : 451 - 453
  • [27] Isolation and characterization of microsatellite loci from the endangered bagrid catfish, Pseudobagrus ichikawai
    Watanabe, K
    Watanabe, T
    Nishida, M
    MOLECULAR ECOLOGY NOTES, 2001, 1 (1-2): : 61 - 63
  • [28] ISOLATION AND CHARACTERIZATION OF NOVEL MICROSATELLITE LOCI FOR THE ENDANGERED ORCHID CYPRIPEDIUM JAPONICUM (ORCHIDACEAE)
    Yamashita, Yumi
    Izuno, Ayako
    Isagi, Yuji
    Kurosawa, Takahide
    Kaneko, Shingo
    APPLICATIONS IN PLANT SCIENCES, 2016, 4 (02):
  • [29] Isolation and characterization of microsatellite loci in the endangered freshwater fish Varicorhinus alticorpus (Cyprinidae)
    Tzen-Yuh Chiang
    Teh-Wang Lee
    Feng-Jiau Lin
    Kuo-Hua Huang
    Hung-Du Lin
    Conservation Genetics, 2008, 9 : 1399 - 1401
  • [30] ISOLATION AND CHARACTERIZATION OF POLYMORPHIC MICROSATELLITE LOCI IN THE ENDANGERED PLANT DIPTERONIA SINENSIS (SAPINDACEAE)
    Su, Hai-Lun
    Li, Zhong-Hu
    Zhao, Peng
    Bai, Guo-Qing
    Zhou, Tian-Hua
    Liu, Zhan-Lin
    Zhao, Gui-Fang
    AMERICAN JOURNAL OF BOTANY, 2012, 99 (10) : E425 - E427