An automated homogeneous liquid-liquid microextraction based on deep eutectic solvent for the HPLC-UV determination of caffeine in beverages

被引：78

作者：

Shishov, Andrey ^{[1
]}

Volodina, Natalia ^{[1
]}

Nechaeva, Daria ^{[1
]}

Gagarinova, Svetlana ^{[1
]}

Bulatov, Andrey ^{[1
]}

机构：

[1] St Petersburg State Univ, Inst Chem, Dept Analyt Chem, SPbSU,SPbU, 7-9 Univ Skaya Nab, St Petersburg 198504, Russia

来源：

MICROCHEMICAL JOURNAL | 2019年 / 144卷

基金：

俄罗斯科学基金会;

关键词：

Homogeneous liquid-liquid microextraction; Deep eutectic solvent; Automation; Flow analysis; HPLC-UV; Caffeine; Soft drinks; PHASE MICROEXTRACTION; ENERGY DRINKS; PERFORMANCE; SAMPLES; EXTRACTION; CHROMATOGRAPHY; VALIDATION; ELECTRODE;

D O I：

10.1016/j.microc.2018.10.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work presents an automated homogeneous liquid-liquid microextraction procedure for separation of caffeine from beverages. The microextraction procedure involved mixing sample and water-miscible deep eutectic solvent (DES, choline chloride with phenol at a 1:3 molar ratio) into a mixing chamber of a flow system resulting formation of homogeneous solution. Injection of aprotic solvent (tetrahydrofuran) into homogeneous sample solution promoted the fine DES droplets formation and caffeine extraction into DES phase separated. The automated homogeneous liquid-liquid microextraction procedure was coupled with a HPLC-UV system. The conditions of caffeine microextraction and its HPLC-UV determination in DES phase were studied and optimized. Under optimal experimental conditions the linear detection range and limit of detection, calculated from a blank test, based on 3a were found to be 0.1-200 mg L-1 and 0.03 mg L-1, respectively. The application of the developed procedure was demonstrated in the determination of caffeine in commercial soft drinks samples.

引用

页码：469 / 473

页数：5

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