Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal

被引：6

作者：

Duan, Zhifeng ^{[1
]}

Senchenkova, Sof'ya N. ^{[2
]}

Guo, Xi ^{[1
]}

Perepelov, Andrei V. ^{[2
]}

Shashkov, Alexander S. ^{[2
]}

Liu, Bin ^{[1
]}

Knirel, Yuriy A. ^{[2
]}

机构：

[1] Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Key Lab Mol Microbiol & Technol,Minist Educ, Tianjin 300457, Peoples R China

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

来源：

CARBOHYDRATE RESEARCH | 2016年 / 430卷

基金：

对外科技合作项目（国际科技项目）; 俄罗斯科学基金会; 中国国家自然科学基金;

关键词：

Escherichia coli; O-polysaccharide; O-antigen; Bacterial polysaccharide structure; O-antigen gene cluster; BIOSYNTHESIS; LIPOPOLYSACCHARIDE; POLYSACCHARIDES;

D O I：

10.1016/j.carres.2016.04.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipopolysaccharide of Escherichin coil O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and H-1 and C-13 NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: 4)-alpha-L-Fucp-(1>6)-alpha-D-Galp-(1>3)-alpha-L-fucp-(1>3)-beta-D-GlcpnAc-(1 3 1 alpha-D-Galp4,6Rpyr where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coil 0156 was analyzed and shown to be consistent with the O-polysaccharide structure. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：24 / 28

页数：5

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