The maize alternative oxidase 1a (Aox1a) gene is regulated by signals related to oxidative stress

被引:42
作者
Polidoros, AN
Mylona, PV
Pasentsis, K
Scandalios, JG
Tsaftaris, AS
机构
[1] Ctr Res & Technol Hellas, Inst Agrobiotechnol, Thermi 57001, Greece
[2] NAGREF, Agr Res Ctr Macedonia Thrace, Thermi, Greece
[3] N Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA
[4] Aristotle Univ Thessaloniki, Dept Genet & Plant Breeding, GR-54006 Thessaloniki, Greece
关键词
alternative oxidase; glutathione S-transferase; reactive oxygen species; nitric oxide; salicylic acid; mitochondrial respiration;
D O I
10.1179/135100005X21688
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We isolated and characterized the expression of Aox1a, a member of the maize alternative oxidase (Aox) small multigene family. Aox1a consists of four exons interrupted by three introns and its promoter harbors diverse stress-specific putative regulatory motifs pointing to complex regulation and response to multiple signals. Responses of Aox1a to such signals were examined and compared with those of maize glutathione S-transferase I ( GstI), a typical oxidative stress inducible gene. Potassium cyanide (KCN) and hydrogen peroxide ( H2O2) induced a rapid increase of the Aox1a and GstI transcripts, which was persisted in prolonged treatment at high H2O2 concentration only for Aox1a. High concentration of salicylic acid ( SA) and salicyl hydroxamic acid ( SHAM) induced Aox1a mRNA only after prolonged exposure, while GstI displayed an early strong induction, which declined thereafter. Nitric oxide ( NO) induced a high increase of Aox1a after prolonged exposure at high concentration, while GstI displayed a weak response. Our results show that multiple signaling pathways, involved in stress responses, also participate and differentially regulate Aox1a and GstI in maize. A ROS-depended signaling event may be involved, suggesting an essential role of Aox1a under oxidative stress in maize.
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页码:71 / 78
页数:8
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