Adsorption equilibrium of α-amylase in aqueous solutions

被引：18

作者：

Bautista, LF ^{[1
]}

Martínez, M ^{[1
]}

Aracil, J ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Chem Engn, E-28040 Madrid, Spain

来源：

AICHE JOURNAL | 1999年 / 45卷 / 04期

关键词：

D O I：

10.1002/aic.690450411

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The influence of pH, ionic strength and temperature on the equilibrium of adsorption of alpha-amylase from Aspergillus oryzae on a hydrophobic (Duolite XAD-761) and an anion-exchange (Duolite A-568)polymer-based adsorbent was studied by moment analysis of the chromatographic peak responses in HPLC. The adsorption isotherms were also measured in batch experiments at different temperatures. Both systems show a nonlinear equilibrium within the concentration range studied, and the results were fitted to both the Langmuir and Freundlich equations. Enthalpies and entropies of adsorption were estimated from the equilibrium adsorption constant obtained both by the moment analysis and by the limit region of the Langmuir isotherm corresponding to Henry's law. The results show that the process of adsorption of alpha-amylase on the hydrophobic resin was exothermic, while the anion exchange process is endothermic.

引用

页码：761 / 768

页数：8

共 41 条

[1]

ADDOYOBO F, 1988, CHEM ENG J, V39, pB17

[2] ISOLATION OF CRYSTALLINE TAKA-AMYLASE A FROM TAKADIASTASE SANKYO [J].

AKABORI, S ;

IKENAKA, T ;

HAGIHARA, B .

JOURNAL OF BIOCHEMISTRY, 1954, 41 (05) :577-582

[3]

ARACIL J, 1993, REV REAL ACAD CIENC, V87, P675

[4]

BAUTISTA LF, 1997, THESIS U COMPLUTENSE

[5] CALCIUM-BINDING IN ALPHA-AMYLASES - AN X-RAY-DIFFRACTION STUDY AT 2.1-A RESOLUTION OF 2 ENZYMES FROM ASPERGILLUS [J].

BOEL, E ;

BRADY, L ;

BRZOZOWSKI, AM ;

DEREWENDA, Z ;

DODSON, GG ;

JENSEN, VJ ;

PETERSEN, SB ;

SWIFT, H ;

THIM, L ;

WOLDIKE, HF .

BIOCHEMISTRY, 1990, 29 (26) :6244-6249

[6]

CASILLAS JL, 1992, J CHEM TECHNOL BIOT, V55, P163

[7]

CASILLAS JL, 1992, THESIS U COMPLUTENSE

[8] A LIQUID-PHASE CHROMATOGRAPHIC STUDY OF SORPTION AND DIFFUSION OF GLUCOSE AND FRUCTOSE IN NAX AND KX ZEOLITE CRYSTALS [J].

CHING, CB ;

RUTHVEN, DM .

ZEOLITES, 1988, 8 (01) :68-73

[9] EVALUATION OF EQUILIBRIUM AND KINETIC-PARAMETERS OF SMALLER MOLECULAR-SIZE AMINO-ACIDS ON KX ZEOLITE CRYSTALS VIA LIQUID-CHROMATOGRAPHIC TECHNIQUES [J].

CHING, CB ;

HIDAJAT, K ;

UDDIN, MS .

SEPARATION SCIENCE AND TECHNOLOGY, 1989, 24 (7-8) :581-597

[10] Adsorption of amino acids on porous polymeric adsorbents .1. Equilibrium [J].

Grzegorczyk, DS ;

Carta, G .

CHEMICAL ENGINEERING SCIENCE, 1996, 51 (05) :807-817

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