Homocysteine Is Biosynthesized from Aspartate Semialdehyde and Hydrogen Sulfide in Methanogenic Archaea

被引：19

作者：

Allen, Kylie D. ^{[1
]}

Miller, Danielle V. ^{[1
]}

Rauch, Benjamin J. ^{[2
]}

Perona, John J. ^{[2
,3
]}

White, Robert H. ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA

[2] Oregon Hlth & Sci Univ, Dept Biochem & Mol Biol, Portland, OR 97239 USA

[3] Portland State Univ, Dept Chem, Portland, OR 97201 USA

来源：

BIOCHEMISTRY | 2015年 / 54卷 / 20期

基金：

美国国家科学基金会;

关键词：

METHANOCOCCUS-JANNASCHII; MECHANISM; REDUCTASE; SULFUR; PROTEINS; CYSTEINE; REVEALS; CLUSTER; COMPLEX; ENZYME;

D O I：

10.1021/acs.biochem.5b00118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthetic route for homocysteine, intermediate in methionine biosynthesis; is unknown in some methanogenic archaea because homologues of the canonical required genes cannot be identified. Here we demonstrate that Methanocaldococcus jannaschii can biosynthesize homocysteine from aspartate semialdehyde and hydrogen sulfide. Additionally, we confirm the genes involved in this new pathway in Methanosarcina acetivorans. A possible series of reactions in which a thioaldehyde is formed and then reduced to a thiol are proposed. This represents a novel route for the biosynthesis of homocysteine and exemplifies unique aspects of Sulfur chemistry occurring in prebiotic environments and in early life forms.

引用

页码：3129 / 3132

页数：4

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