Differential expression of proteins in response to ceramide-mediated stress signal in colon cancer cells by 2-D gel electrophoresis and MALDI-TOF-MS

被引：14

作者：

Fillet, M ^{[1
]}

Cren-Olivé, C

Renert, AF

Piette, J

Vandermoere, F

Rolando, C

Merville, MP

机构：

[1] Univ Liege, CHU B23, Clin Chem Lab, B-4000 Liege, Belgium

[2] Univ Liege, Virol Lab, Ctr Biomed Integrated Genoproteom, B-4000 Liege, Belgium

[3] Univ Sci & Technol Lille, UMR 8009, CNRS, F-59650 Villeneuve Dascq, France

[4] Univ Sci & Technol Lille, IFR 118, ESPRI, INSERM,UPRES EA 1033, F-59650 Villeneuve Dascq, France

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 03期

关键词：

2-D gel; MALDI-TOF-MS; cancer cells; ceramide;

D O I：

10.1021/pr050006t

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative cancer cell proteome analysis is a strategy to study the implication of ceramides in the transmission of stress signals. To better understand the mechanisms by which ceramide regulate some physiological or pathological events and the response to the pharmacological treatment of cancer, we performed a differential analysis of the proteome of HCT-1 16 (human colon carcinoma) cells in response to these substances. We first established the first 2-dimensional map of the HCT-1 16 proteome. Then, HCT116 cell proteome treated or not with C6-ceramicle have been compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization-mass spectrometry and bioinformatic (genomic databases). 2-DE gel analysis revealed more than fourty proteins that were differentially expressed in control cells and cells treated with ceramide. Among them, we confirmed the differential expression of proteins involved in apoptosis and cell adhesion.

引用

页码：870 / 880

页数：11

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