Pseudoruegeria aestuarii sp nov., of the family Rhodobacteraceae, isolated from a tidal flat

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain MME-001(T), was isolated from the tidal flat of Muui-do in the Republic of Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001(T) belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35(T) (98.0% similarity of the 16S rRNA gene). Growth of strain MME-001(T) occurred in the presence of 1.0-7.0% (w/v) NaCl at 15 40 degrees C and pH 7.0-9.0, with optimal growth in the presence of 2.0-3.0% (w/v) NaCl at 25-30 degrees C and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The major cellular fatty acids were C-18 : 1 omega 7c and C-16 : 0. The genomic DNA G+C content was 62 mol%. DNA-DNA hybridization values between strain MME-001(T) and P. sabulilitoris KCTC 42111(T), 'Pseudoruegeria limi' KCTC 32460, Pseudoruegeria lutimaris KCTC 22690(T), Pseudoruegeria aquimaris KCTC 12737(T) and Pseudoruegeriahaliotis KACC 17214(T) was 36 +/- 5, 57 +/- 7, 34 +/- 4, 18 +/- 5 and 21 +/- 3 %, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain MME-001(T) represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain is MME-001(T) (=KCCM 43133(T) =JCM 30751(T)).