Up-regulation of ABCB1/P-glycoprotein by escaping promoter hypermethylation indicates poor prognosis in hematologic malignancy patients with and without bone marrow transplantation

被引：11

作者：

Shi, Cheng-jun ^{[1
]}

Wang, Fang ^{[1
]}

Ren, Mei-feng ^{[1
]}

Mi, Yan-jun ^{[1
]}

Yan, Yan-yan ^{[1
]}

To, Kenneth Kin Wah ^{[3
]}

Dai, Chun-ling ^{[1
]}

Wang, Yan-sheng ^{[2
]}

Chen, Li-ming ^{[1
]}

Tong, Xiu-zhen ^{[2
]}

Liang, Yong-ju ^{[1
]}

Fu, Li-wu ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol So China, Guangzhou 510060, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Hematol, Guangzhou 510060, Guangdong, Peoples R China

[3] Chinese Univ Hong Kong, Sch Pharm, Hong Kong, Hong Kong, Peoples R China

来源：

LEUKEMIA RESEARCH | 2011年 / 35卷 / 01期

关键词：

ATP binding cassette; Multidrug resistance; P-glycoprotein; Methylation; Hematologic malignancy; ACUTE MYELOID-LEUKEMIA; MULTIDRUG-RESISTANCE; P-GLYCOPROTEIN; MDR1; GENE; EXPRESSION; CANCER; CELLS; METHYLATION; PROTEINS; OVEREXPRESSION;

D O I：

10.1016/j.leukres.2010.04.019

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated the correlation between MDR1 promoter methylation status and MDR1 expression in 228 hematologic malignancies patients and 90 healthy controls. High level of MDR1 mRNA correlated to promoter hypomethylation and strongly associated with poor prognosis indicated by 2-year survival rates, poor CR rate (without BMT) and high relapse rate (with BMT). Furthermore, relative luciferase activity of methylated MDR1 at promoter -50 region was significantly higher than that of the unmethylated. In addition, MDR1 in K562 cells elevated significantly after 5-Aza-dC treatment. In summary, MDR1 promoter hypomethylation conferred its up-regulation and indicated poor prognosis in patients with and without BMT. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.

引用

页码：73 / 79

页数：7

共 37 条

[1] K562 - HUMAN ERYTHROLEUKEMIC CELL-LINE
ANDERSSON, LC
NILSSON, K
GAHMBERG, CG
[J]. INTERNATIONAL JOURNAL OF CANCER, 1979, 23 (02) : 143 - 147
[2] Au WY, 2002, HAEMATOLOGICA, V87, P1109
[3] Membrane microparticles mediate transfer of P-glycoprotein to drug sensitive cancer cells
Bebawy, M.
Combes, V.
Lee, E.
Jaiswal, R.
Gong, J.
Bonhoure, A.
Grau, G. E. R.
[J]. LEUKEMIA, 2009, 23 (09) : 1643 - 1649
[4] PROPOSED REVISED CRITERIA FOR THE CLASSIFICATION OF ACUTE MYELOID-LEUKEMIA - A REPORT OF THE FRENCH-AMERICAN-BRITISH COOPERATIVE GROUP
BENNETT, JM
CATOVSKY, D
DANIEL, MT
FLANDRIN, G
GALTON, DAG
GRALNICK, HR
SULTAN, C
[J]. ANNALS OF INTERNAL MEDICINE, 1985, 103 (04) : 620 - 625
[5] EXPRESSION AND ACTIVITY OF P-GLYCOPROTEIN, A MULTIDRUG EFFLUX PUMP, IN HUMAN HEMATOPOIETIC STEM-CELLS
CHAUDHARY, PM
RONINSON, IB
[J]. CELL, 1991, 66 (01) : 85 - 94
[6] CORNWELL MM, 1993, J BIOL CHEM, V268, P19505
[7] Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2
Dai, Chun-ling
Tiwari, Amit K.
Wu, Chung-Pu
Su, Xiao-dong
Wang, Si-Rong
Liu, Dong-geng
Ashby, Charles R., Jr.
Huang, Yan
Robey, Robert W.
Liang, Yong-ju
Chen, Li-ming
Shi, Cheng-Jun
Ambudkar, Suresh V.
Chen, Zhe-Sheng
Fu, Li-wu
[J]. CANCER RESEARCH, 2008, 68 (19) : 7905 - 7914
[8] Damiani D, 2006, HAEMATOLOGICA, V91, P825
[9] Factor VIII bypasses CD91/LRP for endocytosis by dendritic cells leading to T-cell activation
Dasgupta, Suryasarathi
Navarrete, Ana Maria
Andre, Sebastien
Wootla, Bharath
Delignat, Sandrine
Repesse, Yohann
Bayry, Jagadeesh
Nicoletti, Antonino
Saenko, Evgueni L.
d'Oiron, Roseline
Jacquemin, Marc
Saint-Remy, Jean-Marie
Kaveri, Srini, V
Lacroix-Desmazes, Sebastien
[J]. HAEMATOLOGICA, 2007, 93 (01) : 83 - 89
[10] Promoter-specific activation and demethylation by MBD2/demethylase
Detich, N
Theberge, J
Szyf, M
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (39) : 35791 - 35794

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