Induction of dendritic cell maturation by β-glucan isolated from Sparassis crispa

被引：78

作者：

Kim, Hyung Sook ^{[1
,2
]}

Kim, Jee Youn ^{[1
,2
]}

Ryu, Hwa Sun ^{[1
,2
]}

Park, Hyuk-Gu ^{[3
]}

Kim, Yong Ook ^{[4
]}

Kang, Jong Soon ^{[4
]}

Kim, Hwan Mook ^{[4
]}

Hong, Jin Tae ^{[1
,2
]}

Kim, Youngsoo ^{[1
,2
]}

Han, Sang-Bae ^{[1
,2
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea

[2] Chungbuk Natl Univ, Med Res Ctr CICT, Cheongju 361763, Chungbuk, South Korea

[3] Hanabiotech Ltd, Yeoncheon 486860, Kyunggi, South Korea

[4] Korea Res Inst Biosci & Biotechnol, Ochang 363883, Chungbuk, South Korea

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2010年 / 10卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

Sparassis crispa; Sparan; Polysaccharides; Dendritic cells; Toll-like receptor 4; FACTOR GM-CSF; IMMUNOSUPPRESSIVE NETWORKS; HEMATOPOIETIC RESPONSE; CYTOKINE SYNTHESIS; SCG; 1,3-BETA-D-GLUCAN; MICE; DECTIN-1; DIFFERENTIATION; DYSFUNCTION;

D O I：

10.1016/j.intimp.2010.07.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Sparassis crispa is a medicinal mushroom containing high 6-branched 1,3-beta-D-glucan (sparan) content, which exhibits immune-mediated antitumor activity. In the present study, we investigated the stimulating effect of sparan on phenotypic and functional maturation of dendritic cells (DCs). Phenotypic maturation was confirmed by the elevated expressions of CD40, CD80, CD86. and MHC-I/II molecules. Functional activation was proved by increased cytokine production of IL-12, IL-1 beta, TNF-alpha, and IFN-alpha/beta, enhanced IL-2 production and proliferation of allogenic T cells, and decreased endocytosis. The role of toll-like receptor 4 (TLR4) as a membrane receptor of sparan was proved by the impaired maturation of DCs generated from bone marrow cells of tlr4(-/-) knock-out mice and TLR4-mutated C3H/HeJ mice, and by using anti-MD-2/TLR4 neutralizing antibody. Sparan increased phosphorylation of ERK, p38, and INK, and enhanced nuclear translocation of NF-kappa B p50/p65 in DCs. These results indicate that sparan activates DCs via MAPK and NF-kappa B signaling pathways, which are signaling molecules downstream of TLR4. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1284 / 1294

页数：11

共 45 条

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